Budanova E V, Sheina N I
Gig Sanit. 2010 Sep-Oct(5):45-7.
The study was undertaken to evaluate the antibiotic sensitivity of microorganisms that were used in biotechnological production and belonged to different taxons: gram-positive (Bacillus subtilis, Bacillus licheniformis, and Bacillus thuringiensis) and gram-negative (Alcaligenes denitrificans and Pseudomonas caryophylii) bacteria and noncardioform actinomycetes (Rhodococcus erythropolis and Rhodococcus corallinus). The sensitivity of the strains to a range of antibiotics was determined by the agar diffuse method. Industrial strains responded differently to test antibiotics. Gram-positive spore-forming bacteria of the genus Bacillus are most sensitive to antibiotics; the representatives of the genus Rhodococcus are moderately sensitive and the gram-negative bacteria of the genera Pseudomonas and Alcaligenes are resistant to a large number of basic antibiotics and new-generation ones. The findings were made to serve as the basis for developing selective microbiological assays for environment quality control (the air of populated areas or a working zone), which were legally adopted.
本研究旨在评估用于生物技术生产的、属于不同分类群的微生物的抗生素敏感性：革兰氏阳性菌（枯草芽孢杆菌、地衣芽孢杆菌和苏云金芽孢杆菌）、革兰氏阴性菌（反硝化产碱菌和石竹假单胞菌）以及非心脏型放线菌（红平红球菌和珊瑚红球菌）。通过琼脂扩散法测定了这些菌株对一系列抗生素的敏感性。工业菌株对测试抗生素的反应各不相同。芽孢杆菌属的革兰氏阳性产孢细菌对抗生素最为敏感；红球菌属的代表菌株敏感性中等，而假单胞菌属和产碱菌属的革兰氏阴性菌对大量基础抗生素和新一代抗生素具有抗性。这些研究结果为制定法定采用的用于环境质量控制（人口密集地区或工作区的空气）的选择性微生物检测方法奠定了基础。
