Yan Li-hua, Xu Li-zhen, Wang Zhi-min, Zhang Qi-wei, Yang Shi-lin
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Yao Xue Xue Bao. 2010 Dec;45(12):1527-32.
To study the chemical constituents of the stems of Clematis parviloba, six compounds were isolated from a 95% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, ODS, Sephadex LH-20, and semi-preparative HPLC. Two new phenolic glycosides, 2-((E)-3-carboxybut-2-en-yl)-4-hydroxy-3-methyl-phenyl-O-beta-D-glucopyranoside (1) and 4'-hydroxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (2) were isolated, together with a known phenolic glycoside, 4'-hydroxy-3'-methoxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (3) as well as three known megastigmane glycosides, linarionoside A (4), linarionoside C (5), and staphylionoside K (6). Their structures were determined on the basis of spectroscopic analysis and chemical evidence. Among them, compounds 1 and 2 were named as clemaparvilosides A (1) and B (2), respectively, and compounds 3-6 were obtained from Clematis genus for the first time.
为研究毛茛叶铁线莲茎的化学成分，采用硅胶柱色谱、ODS柱色谱、葡聚糖凝胶LH - 20柱色谱及半制备高效液相色谱等多种色谱技术相结合的方法，从95%乙醇提取物中分离得到6个化合物。分离得到2个新的酚苷，即2 - ((E)-3 - 羧基丁 - 2 - 烯基)-4 - 羟基 - 3 - 甲基苯基 - O - β - D - 吡喃葡萄糖苷(1)和4'-羟基苯酚 - β - D - [6 - O -(4"-羟基 - 3",5"-二甲氧基苯甲酸酯)]吡喃葡萄糖苷(2)，以及1个已知酚苷4'-羟基 - 3'-甲氧基苯酚 - β - D - [6 - O -(4"-羟基 - 3",5"-二甲氧基苯甲酸酯)]吡喃葡萄糖苷(3)和3个已知的巨大戟烷苷，即亚麻糖苷A(4)、亚麻糖苷C(5)和葡萄糖苷K(6)。通过光谱分析和化学证据确定了它们的结构。其中，化合物1和2分别命名为毛茛叶铁线莲苷A(1)和B(2)，化合物3 - 6首次从铁线莲属植物中获得。
