Yoshinari N
Department of Periodontology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Aichi Gakuin Daigaku Shigakkai Shi. 1990 Mar;28(1 Pt 2):345-66.
The purpose of the present study was to investigate histologically and histometrically the effect of a long-term methotrexate-induced neutropenia on the periodontal lesion in rats. Forty rats were used in this study. The control side (the interdental area between the left maxillary 1st and 2nd molars) of the rats received no treatment. In the experimental side (the interdental area between the right maxillary 1st and 2nd molars) of the same animals, a suturing nylon thread was inserted into the interdental space between these two molars for 3 weeks to produce a slight inflammation of the gingival tissue. Then, the animals were divided equally into Group A and Group B. Group A received no injection of methotrexate, whereas Group B was injected with 1.0 mg/kg of methotrexate 3 times per week for 1 to 9 weeks. Animals of both groups were killed at 1, 3, 5, 7, and 9 weeks. During the sacrifice, peripheral blood was taken from all animals. White blood cells and neutrophils were counted and the periodontal tissues were examined by light microscopy. 1. Blood findings In Group A, the white blood cell count and neutrophil count did not show any changes for 9 weeks. In Group B, however, the white blood cell count and neutrophil count decreased at 3 to 9 weeks. 2. Histological findings In Group A, the control side did not show any changes for 9 weeks, but the experimental side showed a slight gingival inflammation and a moderate alveolar bone resorption at 7 and 9 weeks. In Group B, the control side was similar to that in Group A for 9 weeks, but the experimental side displayed a slight gingival inflammation and a severe alveolar bone resorption at 7 and 9 weeks. 3. Histometrical findings In Groups A and B, the control sides did not show any changes for 9 weeks in the gingival ressection. However, in both groups, the experimental sides showed an increased gingival ressection from 1 to 9 weeks. In the experimental sides, the gingival ressecion between these two groups did not show any differences statistically. In Groups A and B, the control sides did not show any changes for 9 weeks in alveolar bone loss. However, in both groups, the experimental sides showed increasing alveolar bone loss from 1 to 9 weeks. In the experimental sides, the alveolar bone loss in Group B at 7 and 9 weeks was significantly severer (p less than 0.01) than that in Group A for the same weeks.(ABSTRACT TRUNCATED AT 400 WORDS)
本研究的目的是从组织学和组织计量学角度研究长期甲氨蝶呤诱导的中性粒细胞减少对大鼠牙周病变的影响。本研究使用了40只大鼠。大鼠的对照侧（左上颌第一和第二磨牙之间的牙间隙区域）未接受任何处理。在同一动物的实验侧（右上颌第一和第二磨牙之间的牙间隙区域），将一根缝合尼龙线插入这两颗磨牙之间的牙间隙3周，以引起牙龈组织的轻微炎症。然后，将动物平均分为A组和B组。A组未注射甲氨蝶呤，而B组每周注射1.0mg/kg甲氨蝶呤，共3次，持续1至9周。两组动物在第1、3、5、7和9周处死。处死时，采集所有动物的外周血。计数白细胞和中性粒细胞，并通过光学显微镜检查牙周组织。1.血液检查结果在A组，白细胞计数和中性粒细胞计数在9周内未显示任何变化。然而，在B组，白细胞计数和中性粒细胞计数在第3至9周下降。2.组织学检查结果在A组，对照侧在9周内未显示任何变化，但实验侧在第7和9周出现轻微牙龈炎症和中度牙槽骨吸收。在B组，对照侧在9周内与A组相似，但实验侧在第7和9周出现轻微牙龈炎症和严重牙槽骨吸收。3.组织计量学检查结果在A组和B组中，对照侧的牙龈切除术在9周内未显示任何变化。然而，在两组中，实验侧的牙龈切除术从第1至9周均增加。在实验侧，两组之间的牙龈切除术在统计学上未显示任何差异。在A组和B组中，对照侧的牙槽骨丧失在9周内未显示任何变化。然而，在两组中，实验侧的牙槽骨丧失从第1至9周均增加。在实验侧，B组在第7和9周的牙槽骨丧失比A组在相同周数时明显更严重（p小于0.01）。（摘要截短至400字）
