Yamasaki M
Department of Endodontics, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Aichi Gakuin Daigaku Shigakkai Shi. 1990 Mar;28(1 Pt 2):435-59.
The purpose of the present study was to investigate histologically, histometrically and immunohistochemically the effect of formalin-creosote (FC) on the periapical tissues after pulpectomy in rats. Experiment I. Seventy-five rats were divided into 3 groups. In all Groups, the mesial root of the right mandibular first molar was removed. After pulpectomy, in Group A, a paper point containing saline was inserted into the root canal. In Group B, a paper point containing FC was inserted, whereas, in Group C, FC was applied and then a paper point containing FC was inserted. The access cavity was sealed with amalgam. The animals were killed at 1, 3, 7, 14 and 28 days. The periapical tissues were examined histologically. The vertical length and the area of the apical periodontal ligament were measured histometrically. The inflammatory cells and the fibroblasts per unit area in the apical granulation tissue were counted. Experiment II. Eighteen rats were divided into 3 groups. In all Groups, pulpectomy was performed as in the experiment I. In Group a, a paper point containing saline was inserted into the root canal. In Group b, a paper point containing FC was inserted, whereas, in Group c, FC was applied and then a paper point containing FC was inserted. The animals were killed at 14 and 28 days. The presence of macrophages in the granulation tissue was examined using polyclonal antibody to lysozyme immunohistochemical techniques. The results were as follows. Experiment I. Histologically, in Group A, severe inflammation was observed in the periapical tissues at 1 and 3 days. Inflammation became moderate and fibrous tissue proliferation was observed in the periapical tissues at 7 days. Inflammation became slight and fibrous tissue increased at 14 and 28 days. In Group B, severe inflammation was observed in the periapical tissues at 1 and 3 days. Inflammation became moderate at 7 and 14 days. Resorption of alveolar bone was observed after 7 days. Fibrous tissue proliferation and resorption of the apical root were seen after 14 days. Inflammation became slight at 28 days. In Group C, severe inflammation was found in the periapical tissues at 1, 3 and 7 days. Resorption of alveolar bone was seen after 7 days. Moderate inflammation remained and fibrous tissue proliferation was seen at 14 and 28 days. Resorption of the apical root was observed after 14 days. Histometrically, vertical length and area of the apical periodontal ligament in Groups B and C increased significantly compared with those in Group A at 14 and 28 days.(ABSTRACT TRUNCATED AT 400 WORDS)
本研究的目的是通过组织学、组织计量学和免疫组织化学方法，研究福尔马林 - 杂酚油（FC）对大鼠牙髓切除术后根尖周组织的影响。实验一：75只大鼠分为3组。所有组均切除右侧下颌第一磨牙的近中根。牙髓切除术后，A组将含生理盐水的纸尖插入根管；B组插入含FC的纸尖；C组先应用FC，然后插入含FC的纸尖。用银汞合金封闭髓腔。分别在1、3、7、14和28天处死动物，对根尖周组织进行组织学检查，组织计量学测量根尖牙周膜的垂直长度和面积，计数根尖肉芽组织中单位面积的炎性细胞和成纤维细胞。实验二：18只大鼠分为3组。所有组牙髓切除方法同实验一。a组将含生理盐水的纸尖插入根管；b组插入含FC的纸尖；c组先应用FC，然后插入含FC的纸尖。分别在14和28天处死动物，采用抗溶菌酶多克隆抗体免疫组织化学技术检查肉芽组织中巨噬细胞的存在情况。结果如下。实验一：组织学上，A组在1天和3天时根尖周组织出现严重炎症；7天时炎症减轻，根尖周组织出现纤维组织增生；14天和28天时炎症轻微，纤维组织增多。B组在1天和3天时根尖周组织出现严重炎症；7天和14天时炎症减轻；7天后观察到牙槽骨吸收；14天后出现纤维组织增生和根尖根吸收；28天时炎症轻微。C组在1、3和7天时根尖周组织出现严重炎症；7天后观察到牙槽骨吸收；14天和28天时仍有中度炎症，可见纤维组织增生；14天后观察到根尖根吸收。组织计量学上，B组和C组在14天和28天时根尖牙周膜的垂直长度和面积与A组相比显著增加。（摘要截断于400字）
