Haemotherapy and Haemostais Service, Hospital Clinic, University of Barcelona, Barcelona, Spain.
Int J Lab Hematol. 2011 Apr;33(2):125-32. doi: 10.1111/j.1751-553X.2011.01302.x. Epub 2011 Feb 25.
In recognition of the need for a standardization of the measurement of the erythrocyte sedimentation rate (ESR), the International Council for Standardization in Haematology makes the following recommendations: (i) The reference method for measurement of the ESR should be based on the Westergren method, which is a specific test for the ESR, with modifications, (ii) The reference method for measurement of the ESR should use either whole blood anticoagulated with EDTA and later diluted with sodium citrate or saline (4 : 1) or whole blood anticoagulated with sodium citrate (4 : 1) in Westergren pipettes, (iii) The ESR pipettes can be of glass or plastic (with specific characteristics). It must be colourless; a minimum sedimentation scale of 200 mm, a minimum bore of 2.55 mm, which should be constant within 5%. A protocol for the evaluation of alternative methodologies against the reference method is outlined: The new technologies must be tested over a range of ESR values of 2-120 mm. In this comparison, 95% of the differences should be 5 mm or less, with larger differences associated with higher ESR values. A minimum of 40 samples should be tested in 3 different groups of values: 1-20, 21-60 and more than 60 mm. The statistical methods recommended for ESR evaluations are the coefficient of correlation, the Passing-Bablock regression and the Bland-Altman statistical method. This reference method replaces all earlier standardized and reference methods.
为了规范红细胞沉降率（ESR）的测量，国际血液学标准化委员会提出如下建议：（i）ESR 的参考测量方法应基于 Westergren 法，即 ESR 的特定测试，可进行修改；（ii）ESR 的参考测量方法应使用 EDTA 抗凝的全血，随后用枸橼酸钠或生理盐水（4：1）稀释（或用枸橼酸钠抗凝的全血（4：1））在 Westergren 管中进行；（iii）ESR 管可以是玻璃或塑料（具有特定特性）。它必须是无色的；最小沉淀刻度为 200mm，最小内径为 2.55mm，在 5%以内应保持恒定。概述了针对参考方法评估替代方法的协议：新技术必须在 2-120mm 的 ESR 值范围内进行测试。在此比较中，95%的差异应在 5mm 或以下，较大的差异与较高的 ESR 值相关。应在 3 个不同的数值组（1-20、21-60 和超过 60mm）中测试至少 40 个样本。推荐用于 ESR 评估的统计方法是相关系数、Passing-Bablock 回归和 Bland-Altman 统计方法。这种参考方法取代了所有以前标准化和参考方法。
