Physiologie du Comportement, Université H. Poincaré, 54506 Vandœuvre-les-Nancy, France.
Physiol Behav. 2011 Jun 1;103(3-4):302-7. doi: 10.1016/j.physbeh.2011.02.024. Epub 2011 Feb 23.
This study evaluated behavioural and physiological (hormonal status, muscle structure) affects of prolonged ozone exposure in young females rats. Female Wistar rats were exposed at 0.12ppm of ozone during 6h per day for 15days. Prolonged ozone exposure, though not continuous, produced remarkable behavioural disturbances with an increase in drinking, grooming and resting, and a reduction of rearing, jumping-play and locomotor activities. Besides, ozone exposure was accompanied by increased plasma levels of corticosterone and free triiodothyronine (FT3). Expression of myosin heavy chain (MHC) was significantly affected in three of the five muscles studied. MHC 2B decreased significantly to the benefit of MHC 2A in diaphragm compared to control. MHC 2X increased in Anterior Digastric and decreased in Masseter Superficialis under ozone, to the benefit of MHC 2B in Masseter Superficialis. The plasma corticosterone level increase suggested that prolonged exposure to ozone was stressful. This increase could explain also the increased levels of FT3. Modified respiratory behaviour and hormonal changes both appear to be at the origin of the observed muscular adaptation.
本研究评估了长时间臭氧暴露对年轻雌性大鼠的行为和生理（激素状态、肌肉结构）影响。雌性 Wistar 大鼠每天暴露在 0.12ppm 的臭氧中，每天 6 小时，持续 15 天。尽管不是连续的，但长时间的臭氧暴露会导致明显的行为障碍，表现为饮水量、梳理和休息增加，而觅食、跳跃和运动活动减少。此外，臭氧暴露还伴有血浆皮质酮和游离三碘甲状腺原氨酸（FT3）水平升高。在所研究的五块肌肉中，有三块肌肉的肌球蛋白重链（MHC）表达受到显著影响。与对照组相比，膈肌中 MHC 2B 显著减少，有利于 MHC 2A。在前颌骨肌中，MHC 2X 增加，在表层咬肌中减少，有利于表层咬肌中的 MHC 2B。血浆皮质酮水平升高表明，长时间暴露于臭氧具有应激性。这种增加也可以解释 FT3 水平的升高。呼吸行为的改变和激素变化似乎都是观察到的肌肉适应的原因。
