Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands.
Int J Antimicrob Agents. 2011 Apr;37(4):356-9. doi: 10.1016/j.ijantimicag.2011.01.005. Epub 2011 Feb 25.
Worldwide, resistance of Gram-negative micro-organisms to third-generation cephalosporins and carbapenems owing to β-lactamases is an increasing problem. Although the CTX-M, TEM and SHV extended-spectrum β-lactamases (ESBLs) are most widely disseminated, other β-lactamase families have also recently emerged, such as plasmid-mediated AmpC β-lactamases and carbapenemases. Here we describe a new set of multiplex polymerase chain reactions (PCRs) with one amplification protocol enabling detection of 25 prevalent β-lactamase families, including ESBLs, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.
在全球范围内，由于β-内酰胺酶的存在，革兰氏阴性微生物对第三代头孢菌素和碳青霉烯类药物的耐药性日益成为一个问题。虽然 CTX-M、TEM 和 SHV 型超广谱β-内酰胺酶（ESBLs）的传播最为广泛，但其他β-内酰胺酶家族最近也已出现，如质粒介导的 AmpC β-内酰胺酶和碳青霉烯酶。在这里，我们描述了一组新的多重聚合酶链反应（PCR），采用一种扩增方案，能够检测 25 种常见的β-内酰胺酶家族，包括 ESBLs、碳青霉烯酶、质粒介导的 AmpC β-内酰胺酶和 OXA β-内酰胺酶。
