Tuberculosis Research Unit, Department of Medicine, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, OH 44106-5083, USA.
Tuberculosis (Edinb). 2011 May;91(3):257-9. doi: 10.1016/j.tube.2011.01.004. Epub 2011 Feb 25.
Testing new drugs is critical to improving the treatment of tuberculosis. Quantitative cultures of Mycobacterium tuberculosis on solid media have been used in Phase 1 and 2 trials, but are time and resource intensive. Time to detection (TTD) of growth of M. tuberculosis in automated liquid culture systems is an alternative. TTD has been shown to correlate with CFU in quantitative cultures, and is faster and simpler to perform. We compared TTD in the BACTEC 460 liquid culture system with CFU in a clinical trial that included 110 subjects. Comparing all sputum cultures collected between baseline and 2 months we found a strong negative correlation between log(10) CFU and TTD (rho = -0.91). In addition, when TTD at baseline was compared with 1 and 2 month sputum culture positivity, subjects whose cultures were negative after 1 and 2 months had a significantly longer median baseline TTD compared with subjects whose cultures were positive at 1 and 2 months (5 vs. 3 days and 3 vs. 2 days, respectively). TTD compares closely with CFU and represents a faster, simpler alternative to quantitative cultures.
测试新药物对于改善结核病的治疗至关重要。定量培养固体培养基上的结核分枝杆菌已用于 1 期和 2 期试验，但需要大量的时间和资源。自动化液体培养系统中结核分枝杆菌生长的检测时间 (TTD) 是一种替代方法。已经证明 TTD 与定量培养中的 CFU 相关，并且检测速度更快，操作更简单。我们比较了 BACTEC 460 液体培养系统中的 TTD 与一项临床试验中 110 名受试者的 CFU。对基线至 2 个月期间收集的所有痰培养物进行比较，我们发现 CFU 的对数（10）与 TTD 之间存在强烈的负相关（rho = -0.91）。此外，当将基线时的 TTD 与 1 个月和 2 个月的痰培养阳性进行比较时，与 1 个月和 2 个月时培养阳性的受试者相比，培养在 1 个月和 2 个月时为阴性的受试者的中位基线 TTD 明显更长（分别为 5 天对 3 天和 3 天对 2 天）。TTD 与 CFU 密切相关，是一种更快、更简单的定量培养替代方法。
