[免疫酶测定法（ELISA）在间日疟原虫血清学诊断中的应用]

[The immunoenzymatic assay (ELISA) in the serodiagnosis of Plasmodium vivax].

作者信息

Perez H A, de la Rosa M

机构信息

Instituto Venezolano de Investigaciones Científicas, IVIC, Centro de Microbiología y Biología Celular, Caracas, Venezuela.

出版信息

Rev Inst Med Trop Sao Paulo. 1990 May-Jun;32(3):189-95.

PMID:2135372

Abstract

ELISA was evaluated for the serodiagnosis of Plasmodium vivax using homologous antigen. This was a crude fraction obtained after detergent (NP-40) lysis of human parasitized red blood cells. Antibodies of the classes IgM, IgG, IgA were determined in a pool of eleven sera from patients with P. vivax malaria. The protein A was introduced as secondary probe to screen P. vivax antibodies in 30 sera of patients harbouring a first episode of P. vivax malaria. There was a correlation of 93% with the parasitological diagnosis and the test resulted specific and reproducible.

摘要

使用同源抗原对酶联免疫吸附测定（ELISA）进行评估，以用于间日疟原虫的血清学诊断。这是在用去污剂（NP - 40）裂解人类受疟原虫感染的红细胞后获得的粗提物。在来自间日疟患者的11份血清混合样本中测定了IgM、IgG、IgA类抗体。引入蛋白A作为二抗，用于筛查30例首次感染间日疟原虫患者血清中的间日疟原虫抗体。该检测与寄生虫学诊断的相关性为93%，且结果具有特异性和可重复性。