间日疟原虫热休克蛋白70的特性及其对三日疟血清学诊断价值的评估。
Characterization of Plasmodium vivax heat shock protein 70 and evaluation of its value for serodiagnosis of tertian malaria.
作者信息
Na Byoung-Kuk, Park Jae-Won, Lee Hyeong-Woo, Lin Klin, Kim Seon-Hee, Bae Young-An, Sohn Woon-Mok, Kim Tong-Soo, Kong Yoon
机构信息
Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
出版信息
Clin Vaccine Immunol. 2007 Mar;14(3):320-2. doi: 10.1128/CVI.00424-06. Epub 2007 Jan 17.
Abstract
We have characterized Plasmodium vivax heat shock protein 70 (PvHSP70) and evaluated serodiagnostic applicability of recombinant PvHSP70 (rPvHSP70). In enzyme-linked immunosorbent assays and immunoblot analyses, rPvHSP70 showed high sensitivity (88.8%; 203/228 cases). P. falciparum-infected sera revealed positive reactions (78.8%). The predominant immunoglobulin G (IgG) subclasses were segregated with IgG1 and IgG3.
摘要
我们已对间日疟原虫热休克蛋白70（PvHSP70）进行了特性分析，并评估了重组PvHSP70（rPvHSP70）的血清学诊断适用性。在酶联免疫吸附测定和免疫印迹分析中，rPvHSP70显示出高灵敏度（88.8%；203/228例）。感染恶性疟原虫的血清呈现阳性反应（78.8%）。主要的免疫球蛋白G（IgG）亚类与IgG1和IgG3分离。
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