Service de Microbiologie, Hôpital Robert-Debré (APHP), 75019 Paris, Université Denis Diderot-Paris 7, France.
Diagn Microbiol Infect Dis. 2011 Mar;69(3):245-9. doi: 10.1016/j.diagmicrobio.2010.10.001.
Nonvaccine serotypes are increasingly responsible for pneumococcal empyema. We developed a one-step polymerase chain reaction for identifying all serotypes covered by the 13-valent conjugate vaccine. Over 1 year, we identified serotypes 19A (n = 6), 1 (n = 2), 3 (n = 1) and 7F/A (n = 2), both in culture-positive and culture-negative pleural fluid from 11 children with empyema.
非疫苗血清型导致肺炎球菌性脓胸的比例日益增加。我们开发了一种一步聚合酶链反应，可鉴定 13 价结合疫苗涵盖的所有血清型。在 1 年的时间里，我们在 11 例脓胸患儿的培养阳性和培养阴性胸腔积液中鉴定出了 6 株 19A 型、2 株 1 型、1 株 3 型和 2 株 7F/A 型血清型。
