• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

采用高分辨率熔解分析技术快速、简便、低成本鉴定念珠菌属种。

Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis.

机构信息

Mycoses Unit, Department of Infectious, Parasitic and Immune-mediated Disease, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.

出版信息

Diagn Microbiol Infect Dis. 2011 Mar;69(3):283-5. doi: 10.1016/j.diagmicrobio.2010.10.003.

DOI:10.1016/j.diagmicrobio.2010.10.003
PMID:21353953
Abstract

The feasibility of using high-resolution melting analysis (HRMA) was examined for its rapid and simple detection of 5 clinically relevant Candida species (C. albicans, C. glabrata, C. kefyr, C. parapsilosis, and C. guilliermondii). HRMA was able to differentiate clinical Candida species and resulted in being sensitive, reproducible, and inexpensive.

摘要

高分辨率熔解曲线分析(HRMA)的快速简便检测 5 种临床相关念珠菌(白念珠菌、近平滑念珠菌、近平滑念珠菌、光滑念珠菌和格特隐球菌)的可行性进行了研究。HRMA 能够区分临床念珠菌种,且具有敏感性、重现性和经济性。

相似文献

1
Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis.采用高分辨率熔解分析技术快速、简便、低成本鉴定念珠菌属种。
Diagn Microbiol Infect Dis. 2011 Mar;69(3):283-5. doi: 10.1016/j.diagmicrobio.2010.10.003.
2
The development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis.一种利用高分辨率熔解曲线分析对医学上重要的真菌病原体进行物种鉴定和基因分型的快速基因方法的开发与验证。
Mol Oral Microbiol. 2014 Jun;29(3):117-30. doi: 10.1111/omi.12050. Epub 2014 Apr 12.
3
Real-time LightCycler polymerase chain reaction and melting temperature analysis for identification of clinically important Candida spp.实时荧光聚合酶链反应和熔点温度分析鉴定临床重要念珠菌属。
J Microbiol Immunol Infect. 2009 Aug;42(4):290-5.
4
PCR-based identification of pathogenic Candida species using primer mixes specific to Candida DNA topoisomerase II genes.使用针对念珠菌DNA拓扑异构酶II基因的引物混合物,基于聚合酶链反应(PCR)鉴定致病性念珠菌物种。
Yeast. 2002 Aug;19(11):973-89. doi: 10.1002/yea.892.
5
DNA probes for the rapid identification of medically important Candida species using a multianalyte profiling system.使用多分析物谱系统快速鉴定医学上重要念珠菌属物种的DNA探针。
FEMS Immunol Med Microbiol. 2006 Mar;46(2):244-50. doi: 10.1111/j.1574-695X.2005.00031.x.
6
Comparison of phenotypic methods for the identification of Candida dubliniensis.比较鉴定都柏林念珠菌表型方法。
J Microbiol Immunol Infect. 2010 Apr;43(2):147-54. doi: 10.1016/S1684-1182(10)60023-0.
7
A rapid PCR-based method for identification of four important Candida species.一种基于聚合酶链反应的快速方法用于鉴定四种重要念珠菌属菌种。
New Microbiol. 2005 Jul;28(3):245-50.
8
Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species.使用变性高效液相色谱法快速检测和鉴定七种念珠菌属菌种。
J Clin Microbiol. 2005 Dec;43(12):5912-5. doi: 10.1128/JCM.43.12.5912-5915.2005.
9
Study of molecular epidemiology of candidiasis in portugal by PCR fingerprinting of Candida clinical isolates.通过对念珠菌临床分离株进行PCR指纹图谱分析研究葡萄牙念珠菌病的分子流行病学
J Clin Microbiol. 2004 Dec;42(12):5899-903. doi: 10.1128/JCM.42.12.5899-5903.2004.
10
PCR-AGE, automated and manual methods to identify Candida strains from veterinary sources: a comparative approach.PCR-AGE、自动和手动方法鉴定兽医来源的念珠菌菌株:一种比较方法。
Vet Microbiol. 2009 Nov 18;139(3-4):318-22. doi: 10.1016/j.vetmic.2009.06.031. Epub 2009 Jun 24.

引用本文的文献

1
Development of a Sensitive and Specific Novel qPCR Assay for Simultaneous Detection and Differentiation of Mucormycosis and Aspergillosis by Melting Curve Analysis.通过熔解曲线分析同时检测和鉴别毛霉病和曲霉病的灵敏特异新型定量聚合酶链反应检测方法的开发
Front Fungal Biol. 2022 Jan 24;2:800898. doi: 10.3389/ffunb.2021.800898. eCollection 2021.
2
Molecular identification of Candida isolates by Real-time PCR-high-resolution melting analysis and investigation of the genetic diversity of Candida species.实时 PCR-高分辨率熔解分析鉴定念珠菌分离株及念珠菌种的遗传多样性研究。
J Clin Lab Anal. 2020 Oct;34(10):e23444. doi: 10.1002/jcla.23444. Epub 2020 Jul 12.
3
Rapid identification of clinical common invasive fungi via a multi-channel real-time fluorescent polymerase chain reaction melting curve analysis.
通过多通道实时荧光聚合酶链反应熔解曲线分析快速鉴定临床常见侵袭性真菌
Medicine (Baltimore). 2020 Feb;99(7):e19194. doi: 10.1097/MD.0000000000019194.
4
Marked Strains for Competition Experiments and Bayesian Methods for Fitness Estimates.用于竞争实验的标记菌株和用于适合度估计的贝叶斯方法。
G3 (Bethesda). 2020 Apr 9;10(4):1261-1270. doi: 10.1534/g3.119.400632.
5
Evaluation of a Novel Mitochondrial Pan- Marker for the Detection, Identification, Quantification, and Growth Stage Determination of Mucormycetes.一种用于毛霉菌检测、鉴定、定量及生长阶段判定的新型线粒体全标记物的评估
J Fungi (Basel). 2019 Oct 11;5(4):98. doi: 10.3390/jof5040098.
6
High incidence of invasive fungal infection during acute myeloid leukemia treatment in a resource-limited country: clinical risk factors and treatment outcomes.在资源有限的国家,急性髓细胞白血病治疗期间侵袭性真菌感染的发生率较高:临床危险因素和治疗结果。
Support Care Cancer. 2019 Sep;27(9):3613-3622. doi: 10.1007/s00520-019-04720-5. Epub 2019 Jun 5.
7
Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.酵母感染的分子诊断新趋势:从 PCR 到 NGS。
FEMS Microbiol Rev. 2019 Sep 1;43(5):517-547. doi: 10.1093/femsre/fuz015.
8
High-resolution melting analysis assay for identification of Fonsecaea species.用于鉴定瓶霉属物种的高分辨率熔解分析检测法
J Clin Lab Anal. 2018 Feb;32(2). doi: 10.1002/jcla.22257. Epub 2017 May 22.
9
Dynamic time warping assessment of high-resolution melt curves provides a robust metric for fungal identification.高分辨率熔解曲线的动态时间规整评估为真菌鉴定提供了一种可靠的指标。
PLoS One. 2017 Mar 6;12(3):e0173320. doi: 10.1371/journal.pone.0173320. eCollection 2017.
10
New Panfungal Real-Time PCR Assay for Diagnosis of Invasive Fungal Infections.用于诊断侵袭性真菌感染的新型泛真菌实时荧光定量PCR检测法
J Clin Microbiol. 2016 Dec;54(12):2910-2918. doi: 10.1128/JCM.01580-16. Epub 2016 Sep 14.