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使用变性高效液相色谱法快速检测和鉴定七种念珠菌属菌种。

Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species.

作者信息

Goldenberg Oliver, Herrmann Stefanie, Adam Thomas, Marjoram Gina, Hong George, Göbel Ulf B, Graf Barbara

机构信息

Institute of Microbiology and Hygiene, Charité Universitaetsmedizin Berlin, Campus Charité Mitte, Dorotheenstrasse 96, D-10117 Berlin, Germany.

出版信息

J Clin Microbiol. 2005 Dec;43(12):5912-5. doi: 10.1128/JCM.43.12.5912-5915.2005.

DOI:10.1128/JCM.43.12.5912-5915.2005
PMID:16333075
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1317168/
Abstract

A novel denaturing high-performance liquid chromatography (DHPLC)-based technique allows rapid high-resolution analysis of PCR products. We used this technique for unequivocal molecular identification of seven Candida species. We show the application of this PCR/DHPLC approach for direct detection and identification of yeast species from blood cultures and for detection of Candida colonization in the gastrointestinal tract of allogeneic transplant patients.

摘要

一种基于新型变性高效液相色谱（DHPLC）的技术可对聚合酶链反应（PCR）产物进行快速的高分辨率分析。我们运用该技术对七种念珠菌进行明确的分子鉴定。我们展示了这种PCR/DHPLC方法在直接检测和鉴定血培养中的酵母菌种以及检测异基因移植患者胃肠道念珠菌定植方面的应用。

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Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species.使用变性高效液相色谱法快速检测和鉴定七种念珠菌属菌种。
J Clin Microbiol. 2005 Dec;43(12):5912-5. doi: 10.1128/JCM.43.12.5912-5915.2005.
2
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本文引用的文献

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A one-year survey of candidemia in Belgium in 2002.2002年比利时念珠菌血症的一项为期一年的调查。
Epidemiol Infect. 2004 Dec;132(6):1175-80. doi: 10.1017/s095026880400295x.
2
Rapid and simple differentiation of C. dubliniensis from C. albicans.
Diagn Microbiol Infect Dis. 2004 Feb;48(2):149-51. doi: 10.1016/j.diagmicrobio.2003.09.016.
3
Identification of clinically relevant yeasts by PCR/RFLP.通过聚合酶链反应/限制性片段长度多态性分析鉴定临床相关酵母菌
J Microbiol Methods. 2004 Feb;56(2):201-11. doi: 10.1016/j.mimet.2003.10.007.
4
Culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients.肾移植受者泌尿生殖道中致病细菌和多微生物感染的非培养法鉴定
J Clin Microbiol. 2003 Dec;41(12):5500-10. doi: 10.1128/JCM.41.12.5500-5510.2003.
5
The use of denaturing high-performance liquid chromatography (DHPLC) for the analysis of genetic variations: impact for diagnostics and pharmacogenetics.变性高效液相色谱法（DHPLC）在基因变异分析中的应用：对诊断和药物遗传学的影响
Clin Chem Lab Med. 2003 Apr;41(4):452-61. doi: 10.1515/CCLM.2003.068.
6
Prevalence of Candida species, including Candida dubliniensis, in Singapore.新加坡念珠菌属（包括都柏林念珠菌）的流行情况。
J Clin Microbiol. 2003 Jan;41(1):472-4. doi: 10.1128/JCM.41.1.472-474.2003.
7
Denaturing HPLC for identifying bacteria.用于鉴定细菌的变性高效液相色谱法。
Biotechniques. 2002 Aug;33(2):386-8, 390-1. doi: 10.2144/02332rr05.
8
Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2).通过对扩增的内部转录rRNA间隔区2（ITS2）区域进行荧光片段长度分析来鉴定临床上重要酵母菌种的培养分离株。
BMC Microbiol. 2002 Jul 25;2:21. doi: 10.1186/1471-2180-2-21.
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