Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan.
FEBS Lett. 2011 May 20;585(10):1495-502. doi: 10.1016/j.febslet.2011.02.025. Epub 2011 Feb 25.
The genome of the unicellular green alga Chlamydomonas reinhardtii has both plant-like and animal-like genes. It is of interest to know which types of clock genes this alga has. Recent forward and reverse genetic studies have revealed that its clock has both plant-like and algal clock components. In addition, since C. reinhardtii is a useful model organism also called "green yeast", the identification of clock genes will make C. reinhardtii a powerful model for studying the molecular basis of the eukaryotic circadian clock. In this review, we describe our forward genetic approach in C. reinhardtii and discuss some recent findings about its circadian clock.
单细胞绿藻莱茵衣藻的基因组既有植物样基因，也有动物样基因。了解这种藻类具有哪些类型的生物钟基因是很有意义的。最近的正向和反向遗传研究表明，它的生物钟既有植物样生物钟成分，也有藻类生物钟成分。此外，由于莱茵衣藻也是一种有用的模式生物，也被称为“绿色酵母”，因此鉴定生物钟基因将使莱茵衣藻成为研究真核生物生物钟分子基础的强大模型。在这篇综述中，我们描述了我们在莱茵衣藻中的正向遗传学方法，并讨论了其生物钟的一些最新发现。
