Research Center of Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, PR China.
J Pharm Biomed Anal. 2011 Jun 1;55(3):391-6. doi: 10.1016/j.jpba.2011.01.016. Epub 2011 Jan 22.
Gas chromatography fingerprint of Scutellaria barbata D. Don (SB) from different origins was studied by gas chromatography-mass spectrometry (GC-MS) and related Chemometrics methods. The constituents of essential oil of Scutellaria barbata D. Don (SB) and its two adulterants Oldenlandia diffusa (OD) and Lobelia chinensis Lour (LCL) were analyzed and compared, 50, 36 and 38 components were identified from SB, OD and LCL, respectively, there were 16 and 18 common components between SB and OD, SB and LCL. Nine different samples collected from different producing areas of SB were studied. The relative standard deviations (RSDs) of retention time and peak area of each component were less than 0.2% and 6%, respectively. The number of common peaks was up to 52 and the un-common peak area was less than 10%. The similarity and difference among SB and its adulterants were also evaluated by correlation coefficient similarity analysis and principal component analysis, the result showed that developed fingerprint characterize the SB from different producing areas and it is useful and feasible for the discrimination of its adulterants.
采用气相色谱-质谱联用技术（GC-MS）结合化学计量学方法对不同产地的半枝莲药材进行气相色谱指纹图谱研究。分析比较了半枝莲及其两种混淆品白花蛇舌草、山梗菜的挥发油成分，分别从中鉴定出 50、36 和 38 个化学成分，半枝莲与白花蛇舌草、半枝莲与山梗菜共有化学成分 16 和 18 个。对 9 个不同产地的半枝莲药材进行研究，各成分的保留时间和峰面积的相对标准偏差（RSD）分别小于 0.2%和 6%。共有峰数多达 52 个，非共有峰面积小于 10%。采用相关系数相似度分析和主成分分析评价半枝莲及其混淆品的相似性和差异，结果表明，所建立的指纹图谱能够很好地区分不同产地的半枝莲，并且对半枝莲的混淆品具有较好的鉴别能力。
