Hirata I, Hayashi K, Asada S, Miyoshi H, Iwakoshi K, Ohshiba S
Second Department of Internal Medicine, Osaka Medical College, Japan.
Bull Osaka Med Coll. 1990 Nov;36(1-2):47-55.
The natural killer (NK) activity and NK cell subsets in peripheral blood were evaluated in patients with inflammatory bowel disease (IBD), by using 51Cr release cytotoxicity assay and two color flowcytometry analysis. The peripheral blood NK activity was significantly lower in IBD than that in normal controls. This decrease of the NK activity was independent on the disease activity of IBD but dependent on the steroid medication. The total population of NK cells (Leu 7+ or 11c+ cells) did not change in IBD as compared with those of normal controls. However, the proportions of Leu7+11c- cells and Leu7-11c+ cells in IBD were higher and lower, respectively as compared with those of normal controls. These findings suggest that the maturation step of NK cell lineages might be impaired in IBD. The decrease of NK activity in IBD was supported by the change in the proportion of NK cell subsets described above. It was thought that such a change in NK cell subsets might be a specific finding in IBD patients, because it was not observed in patients with non-IBD colitis.
采用51Cr释放细胞毒性试验和双色流式细胞术分析，对炎症性肠病（IBD）患者外周血中的自然杀伤（NK）活性和NK细胞亚群进行了评估。IBD患者外周血NK活性显著低于正常对照组。NK活性的降低与IBD的疾病活动无关，但与类固醇药物治疗有关。与正常对照组相比，IBD患者NK细胞总数（Leu 7+或11c+细胞）没有变化。然而，与正常对照组相比，IBD患者中Leu7+11c-细胞和Leu7-11c+细胞的比例分别更高和更低。这些发现表明，IBD患者NK细胞谱系的成熟步骤可能受损。上述NK细胞亚群比例的变化支持了IBD患者NK活性的降低。人们认为，NK细胞亚群的这种变化可能是IBD患者的一个特异性表现，因为在非IBD结肠炎患者中未观察到这种变化。
