Brown T E, Bankhurst A D, Strickland R G
J Clin Lab Immunol. 1983 Jul;11(3):113-7.
Peripheral blood natural killer (NK) cell activity and lymphocyte subpopulation profiles were studied in 23 patients with inflammatory bowel disease (IBD) and in 16 healthy control subjects. NK activity was assessed by a 4-hr 51Cr release assay using K-562 erythroleukemia cell line targets. Both monocyte depleted and nondepleted lymphocyte preparations were tested using 50:1 and 25:1 effector to target cell ratios. Lymphocyte subpopulation profiles were determined by incubation of cell suspensions with the monoclonal anti-lymphocyte antibodies (OK-M1, OK-T4, Leu-4 and Leu-2) labeling with goat anti-mouse FITC and sorting by flow cytometry on the fluorescence-activated cell sorter (FACS). NK activity in patients with IBD showed wide variation, but mean activity was not significantly different from normal controls. Analysis of patient subgroups by disease activity, disease type, duration or treatment also failed to reveal significant differences from controls. Parallel measurements of peripheral blood lymphocyte subpopulations using these phenotypic markers revealed no significant differences between patients with IBD and control subjects.
对23例炎症性肠病（IBD）患者和16名健康对照者的外周血自然杀伤（NK）细胞活性及淋巴细胞亚群分布进行了研究。使用K-562红白血病细胞系靶细胞，通过4小时51Cr释放试验评估NK活性。使用50:1和25:1的效应细胞与靶细胞比例，对单核细胞去除和未去除的淋巴细胞制剂都进行了检测。通过将细胞悬液与单克隆抗淋巴细胞抗体（OK-M1、OK-T4、Leu-4和Leu-2）孵育，用山羊抗小鼠FITC标记并在荧光激活细胞分选仪（FACS）上进行流式细胞术分选，来确定淋巴细胞亚群分布。IBD患者的NK活性显示出很大差异，但平均活性与正常对照组无显著差异。按疾病活动度、疾病类型、病程或治疗对患者亚组进行分析，也未发现与对照组有显著差异。使用这些表型标志物对外周血淋巴细胞亚群进行的平行测量显示，IBD患者与对照者之间无显著差异。
