诱导和鉴定鼠源和人源小胶质细胞细胞外诱捕网的方案。
Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells.
机构信息
Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Karolinska Institute and Scilifelab, Stockholm, Sweden.
出版信息
STAR Protoc. 2021 Jul 20;2(3):100678. doi: 10.1016/j.xpro.2021.100678. eCollection 2021 Sep 17.
Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines. For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021).
细胞外陷阱 (ETs) 由去凝聚的染色质组成，并嵌入各种抗菌蛋白，如髓过氧化物酶和组蛋白。最近，我们报道多巴胺 (DA) 以不依赖于细胞死亡、活性氧和肌动蛋白聚合的方式诱导 BV2 小胶质细胞系和原代成人小胶质细胞中的 ETs。本方案详细介绍了如何在 BV2 小胶质细胞和人小胶质细胞中表征 DA 诱导的 ETs。用于表征 ETs 的方案也可用于其他贴壁细胞系。有关使用和执行本方案的完整详细信息，请参阅 Agrawal 等人。（2021 年）。
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