Center for Clinical Spectroscopy, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, 4 Blackfan St, HIM 8-817, Boston, MA 02115, USA.
Radiology. 2011 May;259(2):540-9. doi: 10.1148/radiol.11101123. Epub 2011 Feb 25.
To develop an in vivo two-dimensional localized correlation spectroscopy technique with which to monitor the biochemistry of the human brain and the pathologic characteristics of diseases in a clinically applicable time, including ascertainment of appropriate postprocessing parameters with which to allow diagnostic and prognostic molecules to be measured, and to investigate how much of the chemical information, known to be available from malignant cultured cells, could be recorded in vivo from human brain.
The study was approved by the institutional review board and was compliant with HIPAA. With use of a 3.0-T clinical magnetic resonance (MR) unit and a 32-channel head coil, localized correlation spectroscopy was performed in six healthy control subjects and six patients with glioblastoma multiforme (GBM) with an acquisition time of 11 minutes. Two-dimensional spectra were processed and analyzed and peak volume ratios were tabulated. The data used were proved to be normally distributed by passing the Shapiro-Wilk normality test. The first row of the spectra was extracted to examine diagnostic features. The pathologic characteristics and grade of each GBM were determined after biopsy or surgery. Statistically significant differences were assessed by using a t test.
The localized correlation spectroscopy method assigned biochemical species from the healthy human brain. The correlation spectra of GBM were of sufficiently high quality that many of the cross peaks, recorded previously from malignant cell models in vitro, were observed, demonstrating a statistically significant difference (P < .05) between the cross peak volumes measured for healthy subjects and those with GBM (which include lipid, alanine, N-acetylaspartate, γ-aminobutyric acid, glutamine and glutamate, glutathione, aspartate, lysine, threonine, total choline, glycerophosphorylcholine, myo-inositol, imidazole, uridine diphosphate glucose, isocitrate, lactate, and fucose). The first row of the spectra was found to contain diagnostic features.
Localized correlation spectroscopy of the human brain at 3.0 T with use of a 32-channel head coil was performed in 11 minutes and provided information about neurotransmitters, metabolites, lipids, and macromolecules. The method was able to help differentiate healthy brain from the biochemical signature of GBM in vivo. This method may, in the future, reduce the need for biopsy and is now applicable for the study of selected neurologic diseases.
开发一种可用于实时监测人脑生物化学变化及疾病病理特征的体内二维局域相关波谱技术，包括确定合适的后处理参数，以测量诊断和预后分子，并研究从人体大脑中记录的已知可从恶性培养细胞获得的化学信息有多少。
本研究经机构审查委员会批准，并符合 HIPAA 规定。使用 3.0T 临床磁共振（MR）仪和 32 通道头部线圈，对 6 名健康对照者和 6 名多形性胶质母细胞瘤（GBM）患者进行局域相关波谱分析，采集时间为 11 分钟。对二维谱进行处理和分析，并列出峰体积比。通过 Shapiro-Wilk 正态性检验证明所使用的数据呈正态分布。提取谱的第一行来检查诊断特征。通过活检或手术确定每个 GBM 的病理特征和分级。采用 t 检验评估统计学差异。
局域相关波谱法可对健康人脑的生化物质进行赋值。GBM 的相关谱质量足够高，可观察到以前在体外恶性细胞模型中记录到的许多交叉峰，表明健康受试者与 GBM 患者的交叉峰体积（包括脂质、丙氨酸、N-乙酰天冬氨酸、γ-氨基丁酸、谷氨酰胺和谷氨酸、谷胱甘肽、天冬氨酸、赖氨酸、苏氨酸、总胆碱、甘油磷酸胆碱、肌醇、咪唑、尿苷二磷酸葡萄糖、异柠檬酸、乳酸和岩藻糖）之间存在统计学显著差异（P<.05）。还发现谱的第一行包含诊断特征。
在 3.0T 下使用 32 通道头部线圈对人脑进行局域相关波谱分析可在 11 分钟内完成，并提供有关神经递质、代谢物、脂质和大分子的信息。该方法能够帮助区分健康大脑和 GBM 的生化特征，从而实现体内诊断。该方法将来可能减少对活检的需求，目前可用于某些神经疾病的研究。
