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2
Automated sample-scanning methods for radiation damage mitigation and diffraction-based centering of macromolecular crystals.自动化样品扫描方法在减少辐射损伤和基于衍射的大分子晶体定位中的应用。
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Beamline AR-NW12A: high-throughput beamline for macromolecular crystallography at the Photon Factory.AR-NW12A 光束线:光子工厂内用于大分子晶体学的高通量光束线。
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本文引用的文献

1
Remote access to crystallography beamlines at SSRL: novel tools for training, education and collaboration.斯坦福同步辐射光源晶体学光束线的远程访问:用于培训、教育和合作的新工具。
J Appl Crystallogr. 2010 Oct 1;43(Pt 5):1261-1270. doi: 10.1107/S0021889810024696. Epub 2010 Aug 3.
2
High-speed crystal detection and characterization using a fast-readout detector.使用快速读出探测器进行高速晶体检测与表征
Acta Crystallogr D Biol Crystallogr. 2010 Sep;66(Pt 9):1032-5. doi: 10.1107/S0907444910028192. Epub 2010 Aug 13.
3
MxCuBE: a synchrotron beamline control environment customized for macromolecular crystallography experiments.MxCuBE:一种为大分子晶体学实验定制的同步加速器光束线控制环境。
J Synchrotron Radiat. 2010 Sep;17(5):700-7. doi: 10.1107/S0909049510020005. Epub 2010 Jul 13.
4
Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection.衍射成像技术:将微束应用于大分子晶体学样品评估和数据收集。
Acta Crystallogr D Biol Crystallogr. 2010 Aug;66(Pt 8):855-64. doi: 10.1107/S0907444910019591. Epub 2010 Jul 9.
5
Rastering strategy for screening and centring of microcrystal samples of human membrane proteins with a sub-10 microm size X-ray synchrotron beam.用于使用尺寸小于10微米的X射线同步加速器光束对人膜蛋白微晶样品进行筛选和定心的光栅扫描策略。
J R Soc Interface. 2009 Oct 6;6 Suppl 5(Suppl 5):S587-97. doi: 10.1098/rsif.2009.0142.focus. Epub 2009 Jun 17.
6
Mini-beam collimator enables microcrystallography experiments on standard beamlines.微型准直器可在标准光束线上进行微晶学实验。
J Synchrotron Radiat. 2009 Mar;16(Pt 2):217-25. doi: 10.1107/S0909049508040612. Epub 2009 Jan 10.
7
New paradigm for macromolecular crystallography experiments at SSRL: automated crystal screening and remote data collection.SSRL大分子晶体学实验的新范式:自动晶体筛选与远程数据收集。
Acta Crystallogr D Biol Crystallogr. 2008 Dec;64(Pt 12):1210-21. doi: 10.1107/S0907444908030564. Epub 2008 Nov 18.
8
Implementation of remote monitoring and diffraction evaluation systems at the Photon Factory macromolecular crystallography beamlines.在光子工厂大分子晶体学光束线实施远程监测和衍射评估系统。
J Synchrotron Radiat. 2008 May;15(Pt 3):296-9. doi: 10.1107/S0909049508004019. Epub 2008 Apr 18.
9
Mail-in data collection at SPring-8 protein crystallography beamlines.日本理化学研究所八重洲同步辐射设施蛋白质晶体学光束线的邮寄数据收集。
J Synchrotron Radiat. 2008 May;15(Pt 3):288-91. doi: 10.1107/S0909049507064679. Epub 2008 Apr 18.
10
A 7μm mini-beam improves diffraction data from small or imperfect crystals of macromolecules.一条7微米的微束可改善来自大分子小晶体或不完美晶体的衍射数据。
Acta Crystallogr D Biol Crystallogr. 2008 Apr;64(Pt 4):425-35. doi: 10.1107/S0907444908001741. Epub 2008 Mar 19.

用于大分子晶体学的JBluIce-EPICS控制系统。

JBluIce-EPICS control system for macromolecular crystallography.

作者信息

Stepanov Sergey, Makarov Oleg, Hilgart Mark, Pothineni Sudhir Babu, Urakhchin Alex, Devarapalli Satish, Yoder Derek, Becker Michael, Ogata Craig, Sanishvili Ruslan, Venugopalan Nagarajan, Smith Janet L, Fischetti Robert F

机构信息

GM/CA-CAT at the APS, Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439, USA.

出版信息

Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):176-88. doi: 10.1107/S0907444910053916. Epub 2011 Feb 15.

DOI:10.1107/S0907444910053916
PMID:21358048
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3046456/
Abstract

The trio of macromolecular crystallography beamlines constructed by the General Medicine and Cancer Institutes Collaborative Access Team (GM/CA-CAT) in Sector 23 of the Advanced Photon Source (APS) have been in growing demand owing to their outstanding beam quality and capacity to measure data from crystals of only a few micrometres in size. To take full advantage of the state-of-the-art mechanical and optical design of these beamlines, a significant effort has been devoted to designing fast, convenient, intuitive and robust beamline controls that could easily accommodate new beamline developments. The GM/CA-CAT beamline controls are based on the power of EPICS for distributed hardware control, the rich Java graphical user interface of Eclipse RCP and the task-oriented philosophy as well as the look and feel of the successful SSRL BluIce graphical user interface for crystallography. These beamline controls feature a minimum number of software layers, the wide use of plug-ins that can be written in any language and unified motion controls that allow on-the-fly scanning and optimization of any beamline component. This paper describes the ways in which BluIce was combined with EPICS and converted into the Java-based JBluIce, discusses the solutions aimed at streamlining and speeding up operations and gives an overview of the tools that are provided by this new open-source control system for facilitating crystallographic experiments, especially in the field of microcrystallography.

摘要

由普通医学与癌症研究所协作访问团队(GM/CA-CAT)在先进光子源(APS)23区建造的三条大分子晶体学光束线,因其出色的光束质量以及测量仅几微米大小晶体数据的能力,需求不断增长。为充分利用这些光束线的先进机械和光学设计,已投入大量精力设计快速、便捷、直观且强大的光束线控制系统,使其能够轻松适应新的光束线发展。GM/CA-CAT光束线控制系统基于EPICS的分布式硬件控制能力、Eclipse RCP丰富的Java图形用户界面、面向任务的理念以及成功的SSRL BluIce晶体学图形用户界面的外观和感觉。这些光束线控制系统具有最少的软件层、广泛使用可采用任何语言编写的插件以及统一的运动控制,允许对任何光束线组件进行实时扫描和优化。本文描述了将BluIce与EPICS结合并转换为基于Java的JBluIce的方式,讨论了旨在简化和加速操作的解决方案,并概述了这个新的开源控制系统提供的用于促进晶体学实验,特别是在微晶学领域的工具。