Department of Biotechnology, Kaohsiung Medical University, and Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
J Agric Food Chem. 2011 Mar 23;59(6):2284-90. doi: 10.1021/jf103488j. Epub 2011 Mar 1.
The anticancer effects of (-)-anonaine were investigated in this current study. (-)-Anonaine at concentration ranges of 50-200 μM exhibited significant inhibition to cell growth and migration activities on human lung cancer H1299 cells at 24 h, albeit cell cycle analyses showed that (-)-anonaine at the above concentration ranges did not cause any significant changes in cell-cycle distributions. Significant nuclear damages of H1299 cells were observed with 10-200 μM (-)-anonaine treatment in a comet assay, whereas higher concentrations (6 and 30 mM) of (-)-anonaine concentrations were required to cause DNA damages in an in vitro plasmid cleavage assay. In summary, our results demonstrated that (-)-anonaine exhibited dose-dependent antiproliferatory, antimigratory, and DNA-damaging effects on H1299 cells. We inferred that (-)-anonaine can cause cell-cycle arrest and DNA damage to hamper the physiological behavior of cancer cells at 72 h, and therefore, it can be useful as one of the potential herbal supplements for chemoprevention of human lung cancer.
本研究旨在探讨（-）-非那嗪的抗癌作用。（-）-非那嗪在 50-200 μM 的浓度范围内，在 24 小时时对人肺癌 H1299 细胞的生长和迁移活动表现出显著的抑制作用，尽管细胞周期分析表明（-）-非那嗪在上述浓度范围内不会引起细胞周期分布的任何显著变化。彗星试验显示，（-）-非那嗪在 10-200 μM 的浓度范围内对 H1299 细胞的核损伤有明显的作用，而在体外质粒切割试验中，需要更高的浓度（6 和 30 mM）的（-）-非那嗪浓度才能引起 DNA 损伤。总之，我们的结果表明，（-）-非那嗪对 H1299 细胞表现出剂量依赖性的增殖抑制、迁移抑制和 DNA 损伤作用。我们推断，（-）-非那嗪可以通过引起细胞周期阻滞和 DNA 损伤来阻碍癌细胞的生理行为，因此它可以作为预防人类肺癌的潜在草药补充剂之一。
