J Biomed Opt. 2011 Feb;16(2):020507. doi: 10.1117/1.3548878.
We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K∕AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.
我们合成了载药聚（乳酸-共-乙醇酸）（PLGA）微球，用于 MCF-10A 人乳腺上皮细胞的图像引导组合表观遗传治疗。LY294002 和尼罗红被包裹在微球中，以实现药物的持续释放和荧光显微镜成像。载药微球通过三步骤结合过程靶向 MCF-10A 细胞，该过程涉及生物素化抗体、链霉亲和素和生物素化微球。LY294002 载药微球和 5-Aza-2-脱氧胞苷被应用于 MCF-10A 细胞，用于组合的 PI3K∕AKT 抑制和脱氧核糖核酸（DNA）去甲基化。我们的研究表明，使用载药多功能可生物降解 PLGA 微球进行疾病靶向和图像引导组合表观遗传治疗具有技术潜力。
