National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
J Biomed Opt. 2011 Feb;16(2):021107. doi: 10.1117/1.3533310.
We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) and, lastly, two-dimensional (checkereboard) microstructure. Through the use of this sample series, a comparison of the focal volume, achievable signal-to-noise, and resulting image contrast is made. The results demonstrate the efficiency and spatial resolution attainable in coherent Raman microscopy relative to spontaneous Raman microscopy. Additionally, we detail potential complications in the interpretation of coherent Raman images of sample fine structure, where contrast is no longer based solely on oscillator concentration but can be influenced by sample microstructure.
我们实验比较了自发拉曼和相干斯托克斯拉曼散射显微镜的性能和图像对比度。我们通过一系列几何形状可控的样品展示了这两种技术之间的差异，这些样品的复杂度从点（针尖阵列）到一维（线光栅），最后是二维（棋盘）微结构。通过使用这个样品系列，对聚焦体积、可实现的信噪比和得到的图像对比度进行了比较。结果表明，与自发拉曼显微镜相比，相干拉曼显微镜在效率和空间分辨率方面具有优势。此外，我们详细介绍了在对样品精细结构的相干拉曼图像进行解释时可能出现的复杂情况，其中对比度不再仅仅基于振子浓度，而是可能受到样品微观结构的影响。
