Programa de Recerca en Càncer, IMIM-Hospital del Mar, E-08003, Barcelona, Spain.
Biochem J. 2011 May 1;435(3):563-8. doi: 10.1042/BJ20102057.
Fibronectins are cell-secreted glycoproteins that modulate cell attachment, spreading, migration, morphology, differentiation and oncogenic transformation. Fibronectin expression is activated during EMT (epithelial-mesenchymal transition) and is a hallmark of mesenchymal cells. It is shown in the present study that a transcription factor previously unrelated with EMT, TFCP2c/LSF/LBP-1c, was translocated to the nucleus and bound to the fibronectin promoter upon EMT induction by Snail1. Consequently, the interference of TFCP2c/LSF/LBP-1c's activity prevented fibronectin expression. Moreover, TFCP2c/LSF/LBP-1c was detected in nuclei of embryonic dermal mesenchymal cells adjacent to the hair bud, a cell population that expresses endogenous nuclear Snail1 and fibronectin. Therefore we indicate a new molecular role for TFCP2c/LSF/LBP-1c in fibronectin expression.
纤连蛋白是细胞分泌的糖蛋白，可调节细胞附着、铺展、迁移、形态、分化和致癌转化。纤连蛋白表达在 EMT（上皮-间充质转化）期间被激活，是间充质细胞的标志。本研究表明，先前与 EMT 无关的转录因子 TFCP2c/LSF/LBP-1c 在 EMT 诱导物 Snail1 的作用下被转位到细胞核，并与纤连蛋白启动子结合。因此，TFCP2c/LSF/LBP-1c 的活性干扰阻止了纤连蛋白的表达。此外，在靠近毛囊的胚胎真皮间充质细胞的核中检测到 TFCP2c/LSF/LBP-1c，该细胞群表达内源性核 Snail1 和纤连蛋白。因此，我们指出了 TFCP2c/LSF/LBP-1c 在纤连蛋白表达中的新的分子作用。
