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稻瘟病菌 fimbrin 在菌丝顶端的极性生长和发病机制过程中组织肌动蛋白网络。

Magnaporthe oryzae fimbrin organizes actin networks in the hyphal tip during polar growth and pathogenesis.

机构信息

State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

出版信息

PLoS Pathog. 2020 Mar 16;16(3):e1008437. doi: 10.1371/journal.ppat.1008437. eCollection 2020 Mar.

DOI:10.1371/journal.ppat.1008437
PMID:32176741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7098657/
Abstract

Magnaporthe oryzae causes rice blast disease, but little is known about the dynamic restructuring of the actin cytoskeleton during its polarized tip growth and pathogenesis. Here, we used super-resolution live-cell imaging to investigate the dynamic organization of the actin cytoskeleton in M. oryzae during hyphal tip growth and pathogenesis. We observed a dense actin network at the apical region of the hyphae and actin filaments originating from the Spitzenkörper (Spk, the organizing center for hyphal growth and development) that formed branched actin bundles radiating to the cell membrane. The actin cross-linking protein Fimbrin (MoFim1) helps organize this actin distribution. MoFim1 localizes to the actin at the subapical collar, the actin bundles, and actin at the Spk. Knockout of MoFim1 resulted in impaired Spk maintenance and reduced actin bundle formation, preventing polar growth, vesicle transport, and the expansion of hyphae in plant cells. Finally, transgenic rice (Oryza sativa) expressing RNA hairpins targeting MoFim1 exhibited improved resistance to M. oryzae infection, indicating that MoFim1 represents an excellent candidate for M. oryzae control. These results reveal the dynamics of actin assembly in M. oryzae during hyphal tip development and pathogenesis, and they suggest a mechanism in which MoFim1 organizes such actin networks.

摘要

稻瘟病菌可引发稻瘟病，但人们对其极性顶端生长和发病过程中肌动蛋白细胞骨架的动态重构知之甚少。在这里，我们使用超分辨率活细胞成像技术研究了稻瘟病菌菌丝顶端生长和发病过程中肌动蛋白细胞骨架的动态组织。我们观察到菌丝顶端区域有密集的肌动蛋白网络，肌动蛋白丝起源于 Spitzenkörper（Spk，菌丝生长和发育的组织中心），形成分支的肌动蛋白束辐射到细胞膜。肌动蛋白交联蛋白 Fimbrin（MoFim1）有助于组织这种肌动蛋白分布。MoFim1 定位于亚顶环的肌动蛋白、肌动蛋白束和 Spk 中的肌动蛋白。MoFim1 的敲除导致 Spk 维持受损和肌动蛋白束形成减少，从而阻止极性生长、囊泡运输和菌丝在植物细胞中的扩展。最后，表达靶向 MoFim1 的 RNA 发夹的转基因水稻（Oryza sativa）对稻瘟病菌的感染表现出增强的抗性，表明 MoFim1 是稻瘟病菌控制的一个极好的候选物。这些结果揭示了稻瘟病菌在菌丝顶端发育和发病过程中肌动蛋白组装的动力学，并提出了 MoFim1 组织这种肌动蛋白网络的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/ac7497f41d24/ppat.1008437.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/c0f6f20d8ec1/ppat.1008437.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/3f43ec2df1ea/ppat.1008437.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/6b6f8432ecea/ppat.1008437.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/4c4bbcd5e469/ppat.1008437.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/5dda1a58c201/ppat.1008437.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/65fa592aada9/ppat.1008437.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/d274b215a1f0/ppat.1008437.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/26b48b335f16/ppat.1008437.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/5861ef6bd153/ppat.1008437.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/ac7497f41d24/ppat.1008437.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/c0f6f20d8ec1/ppat.1008437.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/3f43ec2df1ea/ppat.1008437.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/6b6f8432ecea/ppat.1008437.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/4c4bbcd5e469/ppat.1008437.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/5dda1a58c201/ppat.1008437.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/65fa592aada9/ppat.1008437.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/d274b215a1f0/ppat.1008437.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/26b48b335f16/ppat.1008437.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/5861ef6bd153/ppat.1008437.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c95/7098657/ac7497f41d24/ppat.1008437.g010.jpg

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