使用蚀斑减少中和试验检测俄罗斯远东地区野生鸟类西尼罗河病毒血清阳性率。
Seroprevalence of West Nile virus in wild birds in far eastern Russia using a focus reduction neutralization test.
作者信息
Murata Ryo, Hashiguchi Kazuaki, Yoshii Kentaro, Kariwa Hiroaki, Nakajima Kensuke, Ivanov Leonid I, Leonova Galina N, Takashima Ikuo
机构信息
Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
出版信息
Am J Trop Med Hyg. 2011 Mar;84(3):461-5. doi: 10.4269/ajtmh.2011.09-0714.
West Nile (WN) virus has been spreading geographically to non-endemic areas in various parts of the world. However, little is known about the extent of WN virus infection in Russia. Japanese encephalitis (JE) virus, which is closely related to WN virus, is prevalent throughout East Asia. We evaluated the effectiveness of a focus reduction neutralization test in young chicks inoculated with JE and WN viruses, and conducted a survey of WN infection among wild birds in Far Eastern Russia. Following single virus infection, only neutralizing antibodies specific to the homologous virus were detected in chicks. The neutralization test was then applied to serum samples from 145 wild birds for WN and JE virus. Twenty-one samples were positive for neutralizing antibodies to WN. These results suggest that WN virus is prevalent among wild birds in the Far Eastern region of Russia.
西尼罗河（WN）病毒一直在向世界各非流行地区进行地理扩散。然而，对于俄罗斯境内西尼罗河病毒感染的程度却知之甚少。与西尼罗河病毒密切相关的日本脑炎（JE）病毒在整个东亚地区都很普遍。我们评估了在接种日本脑炎病毒和西尼罗河病毒的幼雏中进行蚀斑减少中和试验的有效性，并对俄罗斯远东地区的野生鸟类进行了西尼罗河病毒感染情况调查。在单一病毒感染后，在雏鸡中仅检测到针对同源病毒的中和抗体。然后将中和试验应用于145只野生鸟类针对西尼罗河病毒和日本脑炎病毒的血清样本。21个样本对西尼罗河病毒的中和抗体呈阳性。这些结果表明，西尼罗河病毒在俄罗斯远东地区的野生鸟类中普遍存在。
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