Kariwa Hiroaki, Murata Ryo, Totani Masashi, Yoshii Kentaro, Takashima Ikuo
Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-Ku, Sapporo 060-0818, Japan.
Int J Environ Res Public Health. 2013 Dec 12;10(12):7144-64. doi: 10.3390/ijerph10127144.
In this review, we discuss the possibility that the glycosylation of West Nile (WN) virus E-protein may be associated with enhanced pathogenicity and higher replication of WN virus. The results indicate that E-protein glycosylation allows the virus to multiply in a heat-stable manner and therefore, has a critical role in enhanced viremic levels and virulence of WN virus in young-chick infection model. The effect of the glycosylation of the E protein on the pathogenicity of WN virus in young chicks was further investigated. The results indicate that glycosylation of the WN virus E protein is important for viral multiplication in peripheral organs and that it is associated with the strong pathogenicity of WN virus in birds. The micro-focus reduction neutralization test (FRNT) in which a large number of serum samples can be handled at once with a small volume (15 μL) of serum was useful for differential diagnosis between Japanese encephalitis and WN virus infections in infected chicks. Serological investigation was performed among wild birds in the Far Eastern region of Russia using the FRNT. Antibodies specific to WN virus were detected in 21 samples of resident and migratory birds out of 145 wild bird samples in the region.
在本综述中，我们探讨了西尼罗河（WN）病毒E蛋白糖基化可能与该病毒致病性增强及更高复制水平相关的可能性。结果表明，E蛋白糖基化使病毒能够以热稳定的方式增殖，因此，在幼雏感染模型中，其对WN病毒血症水平升高及毒力增强起着关键作用。我们进一步研究了E蛋白糖基化对WN病毒在幼雏中致病性的影响。结果表明，WN病毒E蛋白的糖基化对外周器官中的病毒增殖很重要，并且与WN病毒在鸟类中的强致病性相关。微量聚焦减少中和试验（FRNT）可使用小体积（15μL）血清一次性处理大量血清样本，对于鉴别诊断感染幼雏中的日本脑炎和WN病毒感染很有用。我们使用FRNT对俄罗斯远东地区的野生鸟类进行了血清学调查。在该地区145份野生鸟类样本中，有21份留鸟和候鸟样本检测到了WN病毒特异性抗体。
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