Patiris Peter J, Oceguera Leopoldo F, Peck George W, Chiles Robert E, Reisen William K, Hanson Carl V
California Department of Public Health, Viral and Rickettsial Disease Laboratory, Richmond, California 94804, USA.
Am J Trop Med Hyg. 2008 Mar;78(3):434-41.
Adult domestic chickens were infected with West Nile virus (WNV) or St. Louis encephalitis virus (SLEV) and challenged with homologous or heterologous virus at 21 or 56 days postinfection (dpi). Sera were collected at selected time points after infection and assayed by enzyme immunoassay (EIA), plaque reduction neutralization test (PRNT), and a Western blot (WB) alternative to PRNT. EIA results were sensitive and accurate (few false positives) but not specific, requiring a confirmatory test to determine virus infection history. PRNT results generally were specific until challenge, after which test results were frequently equivocal and inadequate to determine first or second infecting virus. WB results confirmed the serologic cross-reactivity between WNV and SLEV envelope protein. Non-structural protein 1 and pre-membrane protein reactivities were highly specific for WNV during SLEV infection, but less specific for SLEV during WNV infection. WB and PRNT specificities were similar for both viruses from 6 to 14 dpi, and sensitivities to WNV were virtually identical.
成年家鸡感染西尼罗河病毒（WNV）或圣路易斯脑炎病毒（SLEV），并在感染后21天或56天用同源或异源病毒进行攻毒。在感染后的选定时间点采集血清，通过酶免疫测定（EIA）、蚀斑减少中和试验（PRNT）以及PRNT的替代方法蛋白质印迹法（WB）进行检测。EIA结果灵敏且准确（假阳性少），但不具有特异性，需要进行确证试验来确定病毒感染史。PRNT结果在攻毒前通常具有特异性，攻毒后试验结果常常模棱两可，不足以确定首次或第二次感染的病毒。WB结果证实了WNV和SLEV包膜蛋白之间的血清学交叉反应性。在SLEV感染期间，非结构蛋白1和前膜蛋白反应性对WNV具有高度特异性，但在WNV感染期间对SLEV的特异性较低。在感染后6至14天，两种病毒的WB和PRNT特异性相似，对WNV的敏感性几乎相同。
