Katara Pramod, Gautam Budhayash, Kuntal Himani, Sharma Vinay
Bioinformation. 2010 Sep 20;5(4):162-5. doi: 10.6026/97320630005162.
microRNAs are small noncoding RNA gene products about 20-24nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. As miRNAs affect the morphology of plants and animals by the posttranscriptional regulation of genes involved in critical developmental events, it has been proposed that precise regulation of miRNAs activity during various stages of growth and in specific cell types is of central importance for normal plant development. In our work we focus on the plant miRNAs and predict the miRNA targets, affected proteins by miRNA and miRNA homologs of Glycine max. Our analyses were based on sequence complementarities between miRNAs and mRNAs. As a result, we predicted 573 targets for 44 mature miRNAs sequences among 69 mature miRNAs sequences were published in database. Study of affected proteins revealed that for very less number of miRNAs, protein products are known and they mostly involved in diverse physiological process like as element of photosynthesis system. Homology analyses for miRNAs suggested that 22 miRNAs of Glycine max show 418 miRNA homologs for different plant species.
微小RNA是一类长度约为20 - 24个核苷酸的小型非编码RNA基因产物，由Dicer从具有特征性发夹二级结构的前体加工而来。由于微小RNA通过对参与关键发育事件的基因进行转录后调控来影响动植物的形态，因此有人提出，在生长的各个阶段以及特定细胞类型中精确调控微小RNA的活性对于植物的正常发育至关重要。在我们的工作中，我们专注于植物微小RNA，并预测大豆的微小RNA靶标、受微小RNA影响的蛋白质以及微小RNA同源物。我们的分析基于微小RNA与信使核糖核酸之间的序列互补性。结果，在数据库中已公布的69个成熟微小RNA序列中，我们为44个成熟微小RNA序列预测了573个靶标。对受影响蛋白质的研究表明，只有极少数微小RNA的蛋白质产物是已知的，它们大多参与各种生理过程，如作为光合作用系统的组成部分。对微小RNA的同源性分析表明，大豆的22个微小RNA显示出针对不同植物物种的418个微小RNA同源物。
