Ku Yee-Shan, Wong Johanna Wing-Hang, Mui Zeta, Liu Xuan, Hui Jerome Ho-Lam, Chan Ting-Fung, Lam Hon-Ming
Center for Soybean Research of State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong.
Department of Computer Science, The University of Hong Kong, Pokfulam, Hong Kong.
Int J Mol Sci. 2015 Oct 15;16(10):24532-54. doi: 10.3390/ijms161024532.
To survive under abiotic stresses in the environment, plants trigger a reprogramming of gene expression, by transcriptional regulation or translational regulation, to turn on protective mechanisms. The current focus of research on how plants cope with abiotic stresses has transitioned from transcriptomic analyses to small RNA investigations. In this review, we have summarized and evaluated the current methodologies used in the identification and validation of small RNAs and their targets, in the context of plant responses to abiotic stresses.
为了在环境中的非生物胁迫下生存,植物通过转录调控或翻译调控触发基因表达重编程,以开启保护机制。目前关于植物如何应对非生物胁迫的研究重点已从转录组分析转向小RNA研究。在本综述中,我们在植物对非生物胁迫响应的背景下,总结并评估了用于鉴定和验证小RNA及其靶标的当前方法。