Liu Zhenli, Liu Yuanyan, Chao Zhimao, Song Zhiqian, Wang Chun, Lu Aiping
Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
Nat Prod Commun. 2011 Jan;6(1):55-8.
The root of Polygonum multiflorum Thunb. (Heshouwu in Chinese) is one of the most popular herbs used in traditional Chinese medicine (TCM). However, after steam processing (Zhi-heshouwu in Chinese), the root is known to have different properties and medicinal values compared with Heshouwu. Eleven volatile Maillard reaction products were identified in the extract of Zhi-heshouwu, but not in that of Heshouwu. The new products were four furanones, two furans, two nitrogen compounds, one pyran, one alcohol and one sulfur compound. The antioxidant activities were compared between the extracts from Zhi-heshouwu and Heshouwu. The results showed that the extract from Zhi-heshouwu presented a higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the extract from Heshouwu, with IC50 values of 0.43 mg/mL and 2.9 mg/mL, respectively (p < 0.05). The hydroxyl radical scavenging activities of the two were similar (IC50 0.98 mg/mL and 1.45 mg/mL, respectively; p > 0.05). 5-Hydroxymethyl-furfural, a main compound in the extract of Zhi-heshouwu, showed IC50 values for scavenging DPPH radicals and hydroxyl radicals of 1.6 mg/mL and 0.24 mg/mL, respectively. The antioxidant activities of the extract from Zhi-heshouwu could partly explain the different therapeutic effects of Heshouwu and Zhi-heshouwu in TCM.
何首乌是传统中药中最常用的草药之一。然而，经过炮制（制何首乌）后，其性质和药用价值与何首乌有所不同。在制何首乌提取物中鉴定出11种挥发性美拉德反应产物，而何首乌提取物中未鉴定出。这些新产物包括4种呋喃酮、2种呋喃、2种含氮化合物、1种吡喃、1种醇和1种含硫化合物。比较了制何首乌提取物和何首乌提取物的抗氧化活性。结果表明，制何首乌提取物的1,1-二苯基-2-苦基肼（DPPH）自由基清除活性高于何首乌提取物，IC50值分别为0.43mg/mL和2.9mg/mL（p<0.05）。两者的羟自由基清除活性相似（IC50分别为0.98mg/mL和1.45mg/mL；p>0.05）。制何首乌提取物中的主要化合物5-羟甲基糠醛清除DPPH自由基和羟自由基的IC50值分别为1.6mg/mL和0.24mg/mL。制何首乌提取物的抗氧化活性可以部分解释何首乌和制何首乌在中医中的不同治疗效果。
