Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
Planta Med. 2011 Nov;77(16):1855-60. doi: 10.1055/s-0030-1271200. Epub 2011 Jun 14.
The dried roots of Polygonum multiflorum Thunb. (Heshouwu) and their processed products (Zhi-heshouwu) are widely used in traditional Chinese medicine, yet their therapeutic effects are different. Previous investigations focused mainly on the differences between Heshouwu and Zhi-heshouwu in the contents of several known compounds. In this study, a rapid resolution liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (RRLC/DAD/ESI-MS(n)) method was developed for the comparative analysis of the components of Heshouwu and Zhi-heshouwu. A total of 23 compounds were identified or tentatively characterized. We found that 16 batches of Heshouwu and 15 batches of Zhi-heshouwu samples shared eight compounds, including gallic acid; 3,5,4'-tetrahydroxylstilbene-2,3-di-O-glucoside, CIS-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, trans-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, emodin-8-O- β-D-glucoside, physcion-8-O- β-D-glucoside, emodin, and physcion. Nevertheless, the relative amounts of gallic acid, emodin, and physcion were very high in Zhi-heshouwu samples compared to those in Heshouwu samples. Six compounds disappeared after processing and were unique for Heshouwu: catechin, flavanol gallate dimer, polygonimitin B, emodin-1-O-glucoside, emodin-8-O-(6'-O-malonyl)-glucoside, and physcion-8-O-(6'-O-malonyl)-glucoside. Three compounds were unique for Zhi-heshouwu: hydroxymaltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one, and 5-hydroxymethyl furfural. These results suggest that the types and relative amounts of the chemical components of Heshouwu and Zhi-heshouwu are different.
制何首乌与何首乌的化学成分比较
何首乌（Polygonum multiflorum Thunb.）的干根及其加工品（制何首乌）在中药中广泛应用，但它们的治疗效果不同。先前的研究主要集中在何首乌和制何首乌在几种已知化合物含量上的差异。本研究采用快速分辨液相色谱-二极管阵列检测/电喷雾串联质谱法（RRLC/DAD/ESI-MS(n)）对何首乌和制何首乌的成分进行比较分析。共鉴定或初步鉴定了 23 种化合物。结果发现，16 批何首乌和 15 批制何首乌样品共享 8 种化合物，包括没食子酸；3,5,4'-四羟基二苯乙烯-2,3-二-O-葡萄糖苷、CIS-2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、反式-2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素和大黄素。然而，制何首乌样品中没食子酸、大黄素和大黄素的相对含量明显高于何首乌样品。经炮制后，6 种化合物消失，且为何首乌特有：儿茶素、黄烷醇二聚体、何首乌甲素、大黄素-1-O-葡萄糖苷、大黄素-8-O-(6'-O-丙二酰基)-葡萄糖苷和大黄素-8-O-(6'-O-丙二酰基)-葡萄糖苷。3 种化合物为制何首乌特有：羟甲基糠醛、2,3-二氢-3,5-二羟基-6-甲基-4(H)-吡喃-4-酮和 5-羟甲基糠醛。这些结果表明，何首乌和制何首乌的化学成分类型和相对含量不同。
