National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1-1 Higashi, Ibaraki, Tsukuba, Japan.
Biotechnol Lett. 2011 Jul;33(7):1429-33. doi: 10.1007/s10529-011-0573-0. Epub 2011 Mar 3.
Of six strains of lactic acid-producing alkaliphilic microorganisms, Halolactibacillus halophilus was most efficient. It produced the highest concentration and yield of lactic acid, with minimal amounts of acetic and formic acid when sucrose and glucose were used as substrate. Mannose and xylose were poorly utilized. In batch fermentation at 30°C, pH 9 with 4 and 8% (w/v) sucrose, lactic acid was produced at 37.7 and 65.8 g l(-1), with yields of 95 and 83%, respectively. Likewise, when 4 and 8% (w/v) glucose were used, 33.4 and 59.6 g lactic acid l(-1) were produced with 85 and 76% yields, respectively. L: -(+)-lactic acid had an optical purity of 98.8% (from sucrose) and 98.3% (from glucose).
在 6 株产乳酸的耐碱嗜热微生物菌株中，嗜盐乳杆菌的效率最高。当以蔗糖和葡萄糖作为底物时，它产生的乳酸浓度和产量最高，而乙酸和甲酸的产量最少。甘露糖和木糖的利用率较低。在 30°C、pH9 的分批发酵中，4%和 8%（w/v）的蔗糖分别产生 37.7 和 65.8 g/L 的乳酸，产率分别为 95%和 83%。同样，当使用 4%和 8%（w/v）的葡萄糖时，分别产生 33.4 和 59.6 g/L 的乳酸，产率分别为 85%和 76%。L: -(+)-乳酸的光学纯度为 98.8%（来自蔗糖）和 98.3%（来自葡萄糖）。
