Casale T B, Ballas Z K, Kaliner M A, Keahey T M
Department of Internal Medicine, Veterans Administration Medical Center, Iowa City, Iowa.
J Allergy Clin Immunol. 1990 Jan;85(1 Pt 1):45-51. doi: 10.1016/0091-6749(90)90219-t.
To understand better the pathogenesis of gram-negative infections, we administered a single intravenous injection of Escherichia coli RE-2 endotoxin (4 ng/kg) to five normal volunteers and periodically measured various host-effector molecules in blood. All subjects had a significant (p less than 0.05) increase in temperature (maximum of 1.3 +/- 0.1 degrees C at 4 hours) and white blood cell count (maximum of 7700 +/- 1800 cells per cubic millimeters at 8 hours). Thymocyte costimulatory activity in blood was markedly increased 2 hours after endotoxin administration but returned to baseline at 4 hours. Blood cortisol and beta-endorphin levels paralleled each other, peaking 4 hours after endotoxin (mean increases of 21 +/- 14 micrograms/dl and p less than 0.01; 18.6 +/- 5.9 pg/ml and p less than 0.05, respectively) and returning to baseline at 8 hours. Blood histamine levels did not significantly change at any time point after endotoxin administration. Since thymocyte costimulatory activity was the first of the measured parameters to achieve peak blood levels, we suggest that endotoxin-induced increases in various cytokines may contribute to the increases in the other measured parameters and thereby play a significant role in the pathogenesis of gram-negative infections.
为了更好地理解革兰氏阴性菌感染的发病机制，我们对5名正常志愿者静脉注射了一次大肠杆菌RE - 2内毒素（4 ng/kg），并定期测量血液中的各种宿主效应分子。所有受试者的体温均显著升高（p小于0.05）（4小时时最高升高1.3±0.1摄氏度），白细胞计数也显著升高（8小时时最高达到每立方毫米7700±1800个细胞）。内毒素注射后2小时，血液中胸腺细胞共刺激活性显著增加，但在4小时时恢复到基线水平。血液中的皮质醇和β-内啡肽水平相互平行，在内毒素注射后4小时达到峰值（平均分别增加21±14微克/分升，p小于0.01；18.6±5.9皮克/毫升，p小于0.05），并在8小时时恢复到基线水平。内毒素注射后任何时间点血液中的组胺水平均无显著变化。由于胸腺细胞共刺激活性是第一个达到血液峰值水平的测量参数，我们认为内毒素诱导的各种细胞因子增加可能导致其他测量参数的增加，从而在革兰氏阴性菌感染的发病机制中发挥重要作用。
