Elin R J, Wolff S M, McAdam K P, Chedid L, Audibert F, Bernard C, Oberling F
J Infect Dis. 1981 Oct;144(4):329-36. doi: 10.1093/infdis/144.4.329.
The properties of a reference bacterial endotoxin prepared from Escherichia coli and its phthalylated derivative were studied in normal human volunteers infected intravenously with the compounds. The minimal pyrogenic dose of the reference endotoxin is about 0.1-0.5 ng/kg. The increase in white blood cell count, absolute granulocyte count, absolute immature granulocyte count, and concentrations of serum amyloid A, cortisol, and growth hormone was directly related to the concentration of reference endotoxin administered. Phthalylated reference endotoxin up to 1,000 ng/kg (at least 500 ng of the patent compound/kg) was administered to normal human volunteers without significant changes in temperature, white blood cell count, absolute granulocyte count, and concentrations of serum amyloid A, cortisol, and growth hormone. Thus, this study defines biologic properties of the new reference bacterial endotoxin in humans and demonstrates effective detoxification by phthalylation of the present compound.
在静脉注射了由大肠杆菌制备的参考细菌内毒素及其邻苯二甲酰化衍生物的正常人类志愿者中，对这些化合物的特性进行了研究。参考内毒素的最小致热剂量约为0.1 - 0.5 ng/kg。白细胞计数、绝对粒细胞计数、绝对未成熟粒细胞计数以及血清淀粉样蛋白A、皮质醇和生长激素浓度的增加与所给予的参考内毒素浓度直接相关。向正常人类志愿者注射高达1000 ng/kg的邻苯二甲酰化参考内毒素（至少500 ng专利化合物/kg），体温、白细胞计数、绝对粒细胞计数以及血清淀粉样蛋白A、皮质醇和生长激素浓度均无显著变化。因此，本研究确定了新型参考细菌内毒素在人体内的生物学特性，并证明了该化合物通过邻苯二甲酰化实现了有效的解毒。
