Khiev Piseth, Oh Sei-Ryang, Chae Hee-Sung, Kwon Ok-Kyoung, Ahn Kyung-Seop, Chin Young-Won, Lee Hyeong-Kyu
University of Science & Technology, Daejeon, Korea.
Chem Pharm Bull (Tokyo). 2011;59(3):382-4. doi: 10.1248/cpb.59.382.
Bioactivity-guided isolation on a n-hexane-soluble fraction of Thyrsanthera suborbicularis led to the isolation of a new rosane-type diterpene, 19-hydroxy-1(10), 15-rosadiene (1), along with three known compounds, taraxerol, acetyl aleuritolic acid, and spathulenol. The structures of isolated compounds were determined by interpretation of NMR spectroscopic data and mass spectrometry. Compound 1 demonstrated significantly inhibitory activity on nitric oxide production in RAW264.7 lipopolysaccharide (LPS)-activated mouse macrophages with an IC(50) value of 2.91 µg/ml via the suppression of inducible nitric oxide synthase (iNOS) mRNA expression.
对圆腺叶藤（Thyrsanthera suborbicularis）正己烷可溶部分进行生物活性导向分离，得到一种新的蔷薇烷型二萜，19-羟基-1(10),15-蔷薇二烯（1），以及三种已知化合物，蒲公英赛醇、乙酰齐墩果酸和匙叶桉油烯醇。通过对核磁共振光谱数据和质谱的解析确定了分离得到化合物的结构。化合物1对RAW264.7脂多糖（LPS）激活的小鼠巨噬细胞中一氧化氮的产生具有显著抑制活性，通过抑制诱导型一氧化氮合酶（iNOS）mRNA表达，其IC(50)值为2.91 µg/ml。
