Lee Byung-Cheon, Kim Hong Bae, Sung Baeckkyoung, Kim Ki Woo, Sohn Jamin, Son Boram, Chang Byung-Joon, Soh Kwang-Sup
Pharmacopuncture Medical Research Institute, Korean Pharmacopuncture Institute, Seoul, Korea.
Cardiology. 2011;118(1):1-7. doi: 10.1159/000323844. Epub 2011 Mar 2.
Although there have been reports on threadlike structures inside the heart, they have received little attention. We aimed to develop a method for observing such structures and to reveal their ultrastructures.
An in situ staining method, which uses a series of procedures of 0.2-0.4% trypan blue spraying and washing, was applied to observe threadlike structures on the surfaces of endocardia. The threadlike structures were isolated and observed by using confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM).
Networks of endocardial vessels (20 μm in thickness) with expansions (40-100 μm in diameter) were visualized; they were movable on the endocardium of the bovine atrium and ventricle. CLSM showed that (1) rod-shaped nuclei were aligned along the longitudinal direction of the endocardial vessel and (2) there were many cells inside the expansion. TEM on the endocardial vessel revealed that (1) there existed multiple lumens (1-7 μm in diameter) and (2) the extracellular matrices mostly consisted of collagen fibers, which were aligned along the longitudinal direction of the endocardial vessel or were locally organized in reticular structures.
We investigated the endocardial circulatory system in bovine cardiac chambers and its ultrastructures, such as nucleic distributions, microlumens, and collagenous extracellular matrices.
尽管已有关于心脏内部丝状结构的报道，但它们很少受到关注。我们旨在开发一种观察此类结构的方法，并揭示其超微结构。
采用原位染色法，即通过一系列0.2 - 0.4%台盼蓝喷雾和冲洗程序，观察心内膜表面的丝状结构。通过共聚焦激光扫描显微镜（CLSM）和透射电子显微镜（TEM）对丝状结构进行分离和观察。
观察到厚度为20μm的心内膜血管网络，其具有直径为40 - 100μm的扩张部分；它们可在牛心房和心室的心内膜上移动。CLSM显示：（1）杆状细胞核沿心内膜血管的纵向排列；（2）扩张部分内部有许多细胞。心内膜血管的TEM显示：（1）存在多个直径为1 - 7μm的管腔；（2）细胞外基质主要由胶原纤维组成，这些胶原纤维沿心内膜血管的纵向排列或局部组织成网状结构。
我们研究了牛心脏腔室的心内膜循环系统及其超微结构，如细胞核分布、微管腔和胶原性细胞外基质。
