Yeon Sun Hee, Kwon O Sang, Cho Seong Jin, Kang Suk Yun, Moon Ji Young, Ryu Yeonhee
KM Fundamental Research Division, Korea institute of Oriental Medicine, Daejon, Korea.
Integr Med Res. 2016 Mar;5(1):56-62. doi: 10.1016/j.imr.2015.12.002. Epub 2015 Dec 17.
The novel threadlike structure is called the primo structure, and studies are conducted through many different approaches. Although various ways of differentiation are currently used, a standard for differentiation is deemed necessary in order to identify the primo structure based on the overall form of the structure. This study aims to explore the differentiation standard through the histological common point of the threadlike structure of rat and swine by using the hematoxylin and eosin (H&E) staining method commonly used for histological research in various structures.
An 8-week old Sprague-Dawley rat and a Yorkshire pig weighing 30-40 kg were used. A total of 65 pieces of rat threadlike structure and 100 pieces of swine threadlike structure were collected after the abdomen was cut and opened. The following three different characteristics were confirmed using the H&E staining method for the collected structures: (1) bright cell availability; (2) cavity availability; and (3) nuclei density.
For the rat threadlike structure, the bright cell (70.5%) and nuclei density (92.6%) were mainly observed; in the swine threadlike structure, however, the bright cell (60.6%) and cavity (67.2%) were mainly observed. The bright cell was confirmed to have been observed in the threadlike structures of both rat and swine.
The bright cell is determined to be the common point in the primo structure. However, further research is deemed necessary in the future as to the functions performed by the characteristic shown by the Primo structure.
这种新型的丝状结构被称为原始结构,并且通过许多不同的方法进行研究。尽管目前使用了各种分化方式,但为了基于结构的整体形态识别原始结构,认为有必要制定一个分化标准。本研究旨在通过使用常用于各种结构组织学研究的苏木精和伊红(H&E)染色方法,探索大鼠和猪丝状结构的组织学共同点,以此来确定分化标准。
使用一只8周龄的斯普拉格-道利大鼠和一头体重30 - 40千克的约克夏猪。在切开并打开腹部后,共收集了65条大鼠丝状结构和100条猪丝状结构。对收集到的结构使用H&E染色方法确认以下三个不同特征:(1)亮细胞的存在情况;(2)腔的存在情况;(3)细胞核密度。
对于大鼠丝状结构,主要观察到亮细胞(70.5%)和细胞核密度(92.6%);然而,在猪丝状结构中,主要观察到亮细胞(60.6%)和腔(67.2%)。已证实在大鼠和猪的丝状结构中均观察到了亮细胞。
亮细胞被确定为原始结构中的共同点。然而,关于原始结构所显示特征的功能,未来仍需进一步研究。