建立并鉴定中国人源肺癌原代细胞系。
Establishment and characterization of primary lung cancer cell lines from Chinese population.
机构信息
Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, China.
出版信息
Acta Pharmacol Sin. 2011 Mar;32(3):385-92. doi: 10.1038/aps.2010.214.
Abstract
AIM
To establish and characterize primary lung cancer cell lines from Chinese population.
METHODS
Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice.
RESULTS
Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations.
CONCLUSION
These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response.
摘要
目的
建立并鉴定中国人源肺癌原代细胞系。
方法
从中国肺癌患者的肺癌标本或胸腔积液中采集样本，在 ACL4 培养基（用于非小细胞肺癌（NSCLC））或 HITES 培养基（用于小细胞肺癌（SCLC））中进行体外培养，培养基中添加 5%FBS。所有细胞系均在培养中维持超过 25 个传代。对这些细胞系中的大多数进一步进行了致癌突变、核型、细胞生长动力学和裸鼠致瘤性分析。
结果
从中国肺癌患者中建立并鉴定了 8 株原代细胞系，包括 7 株 NSCLC 细胞系和 1 株 SCLC 细胞系。发现 5 株 NSCLC 细胞系存在表皮生长因子受体（EGFR）激酶结构域突变。
结论
这些经过良好鉴定的中国人源肺癌原代细胞系为研究肺癌生物学和药物反应中的种族差异提供了独特的平台。
相似文献
1
Establishment and characterization of primary lung cancer cell lines from Chinese population.建立并鉴定中国人源肺癌原代细胞系。
Acta Pharmacol Sin. 2011 Mar;32(3):385-92. doi: 10.1038/aps.2010.214.
2
EGFR mutations in surgically resected fresh specimens from 697 consecutive Chinese patients with non-small cell lung cancer and their relationships with clinical features.697 例连续的中国非小细胞肺癌手术切除新鲜标本中 EGFR 突变及其与临床特征的关系。
Int J Mol Sci. 2013 Dec 17;14(12):24549-59. doi: 10.3390/ijms141224549.
3
RB loss in resistant EGFR mutant lung adenocarcinomas that transform to small-cell lung cancer.对表皮生长因子受体（EGFR）突变的耐药性肺腺癌转化为小细胞肺癌过程中的RB基因缺失。
Nat Commun. 2015 Mar 11;6:6377. doi: 10.1038/ncomms7377.
4
Association between epidermal growth factor receptor gene copy number and ERCC1, BRCA1 protein expression in Chinese patients with non-small cell lung cancer.表皮生长因子受体基因拷贝数与 ERCC1、BRCA1 蛋白表达在非小细胞肺癌中国患者中的相关性。
Med Oncol. 2014 Mar;31(3):803. doi: 10.1007/s12032-013-0803-5. Epub 2014 Jan 23.
5
Genomic Signature of Driver Genes Identified by Target Next-Generation Sequencing in Chinese Non-Small Cell Lung Cancer.中国非小细胞肺癌中靶向下一代测序鉴定的驱动基因的基因组特征。
Oncologist. 2019 Nov;24(11):e1070-e1081. doi: 10.1634/theoncologist.2018-0572. Epub 2019 Mar 22.
6
EGFR over-expression in non-small cell lung cancers harboring EGFR mutations is associated with marked down-regulation of CD82.在携带表皮生长因子受体（EGFR）突变的非小细胞肺癌中，EGFR过表达与CD82的显著下调相关。
Biochim Biophys Acta. 2015 Jul;1852(7):1540-9. doi: 10.1016/j.bbadis.2015.04.020. Epub 2015 Apr 23.
7
Establishment of patient-derived non-small cell lung cancer xenografts as models for the identification of predictive biomarkers.建立患者来源的非小细胞肺癌异种移植模型用于预测生物标志物的鉴定。
Clin Cancer Res. 2008 Oct 15;14(20):6456-68. doi: 10.1158/1078-0432.CCR-08-0138.
8
Establishment and characterization of a panel of human lung cancer cell lines.一组人肺癌细胞系的建立与鉴定
Cancer. 1992 Apr 15;69(8):2064-74. doi: 10.1002/1097-0142(19920415)69:8<2064::aid-cncr2820690811>3.0.co;2-#.
9
Elevated expression of Fn14 in non-small cell lung cancer correlates with activated EGFR and promotes tumor cell migration and invasion.Fn14 在非小细胞肺癌中的高表达与激活的 EGFR 相关，并促进肿瘤细胞迁移和侵袭。
Am J Pathol. 2012 Jul;181(1):111-20. doi: 10.1016/j.ajpath.2012.03.026. Epub 2012 May 23.
10
PLAUR Confers Resistance to Gefitinib Through EGFR/P-AKT/Survivin Signaling Pathway.PLAUR通过EGFR/P-AKT/生存素信号通路赋予对吉非替尼的抗性。
Cell Physiol Biochem. 2018;47(5):1909-1924. doi: 10.1159/000491071. Epub 2018 Jun 29.
引用本文的文献
1
High cereblon expression in neuroendocrine cancer confers vulnerability to GSPT1 molecular glue degrader.神经内分泌癌中高表达的 Cereblon 使细胞对 GSPT1 分子胶降解剂敏感。
Exp Hematol Oncol. 2025 Jun 23;14(1):89. doi: 10.1186/s40164-025-00674-z.
2
[Application and Research Progress of Lung Cancer Organoid in Precision Medicine
for Lung Cancer].肺癌类器官在肺癌精准医学中的应用与研究进展
Zhongguo Fei Ai Za Zhi. 2024 Apr 20;27(4):276-282. doi: 10.3779/j.issn.1009-3419.2024.106.07.
3
Lack of racial and ethnic diversity in lung cancer cell lines contributes to lung cancer health disparities.肺癌细胞系中缺乏种族和民族多样性导致了肺癌健康差异。
Front Oncol. 2023 Nov 1;13:1187585. doi: 10.3389/fonc.2023.1187585. eCollection 2023.
4
SFPQ and Its Isoform as Potential Biomarker for Non-Small-Cell Lung Cancer.SFPQ 及其同种型作为非小细胞肺癌的潜在生物标志物。
Int J Mol Sci. 2023 Aug 6;24(15):12500. doi: 10.3390/ijms241512500.
5
Mechanistic insights into CDCP1 clustering on non-small-cell lung cancer membranes revealed by super-resolution fluorescent imaging.超分辨率荧光成像揭示非小细胞肺癌细胞膜上CDCP1聚集的机制洞察
iScience. 2023 Feb 2;26(3):106103. doi: 10.1016/j.isci.2023.106103. eCollection 2023 Mar 17.
6
The application of patient-derived organoid in the research of lung cancer.患者来源类器官在肺癌研究中的应用。
Cell Oncol (Dordr). 2023 Jun;46(3):503-519. doi: 10.1007/s13402-023-00771-3. Epub 2023 Jan 25.
7
Generation of murine tumour-reactive T cells by co-culturing murine pancreatic cancer organoids and peripheral blood lymphocytes.通过将小鼠胰腺癌类器官与外周血淋巴细胞共培养来生成小鼠肿瘤反应性T细胞。
Biochem Biophys Rep. 2022 Oct 9;32:101365. doi: 10.1016/j.bbrep.2022.101365. eCollection 2022 Dec.
8
SFPQ Promotes Lung Cancer Malignancy Regulation of CD44 v6 Expression.SFPQ促进肺癌恶性进展 CD44 v6表达的调控
Front Oncol. 2022 May 30;12:862250. doi: 10.3389/fonc.2022.862250. eCollection 2022.
9
Establishment and characterization of a novel highly malignant lung cancer cell line ZX2021H derived from a metastatic lymph node lesion.一种源自转移性淋巴结病变的新型高恶性肺癌细胞系ZX2021H的建立与鉴定
Thorac Cancer. 2022 Apr;13(8):1199-1210. doi: 10.1111/1759-7714.14385. Epub 2022 Mar 16.
10
Modeling Clinical Responses to Targeted Therapies by Patient-Derived Organoids of Advanced Lung Adenocarcinoma.利用晚期肺腺癌患者来源的类器官模型来模拟靶向治疗的临床反应。
Clin Cancer Res. 2021 Aug 1;27(15):4397-4409. doi: 10.1158/1078-0432.CCR-20-5026. Epub 2021 Jun 3.
本文引用的文献
1
Lung adenocarcinoma from East Asian never-smokers is a disease largely defined by targetable oncogenic mutant kinases.东亚从不吸烟人群的肺腺癌是一种主要由可靶向的致癌基因突变激酶定义的疾病。
J Clin Oncol. 2010 Oct 20;28(30):4616-20. doi: 10.1200/JCO.2010.29.6038. Epub 2010 Sep 20.
2
Lung cancer cell lines as tools for biomedical discovery and research.肺癌细胞系作为生物医学发现和研究的工具。
J Natl Cancer Inst. 2010 Sep 8;102(17):1310-21. doi: 10.1093/jnci/djq279. Epub 2010 Aug 2.
3
Cancer statistics, 2010.癌症统计数据，2010 年。
CA Cancer J Clin. 2010 Sep-Oct;60(5):277-300. doi: 10.3322/caac.20073. Epub 2010 Jul 7.
4
Spectrum of LKB1, EGFR, and KRAS mutations in chinese lung adenocarcinomas.中国肺腺癌中 LKB1、EGFR 和 KRAS 基因突变谱。
J Thorac Oncol. 2010 Aug;5(8):1130-5. doi: 10.1097/JTO.0b013e3181e05016.
5
Comparable rate of EGFR kinase domain mutation in lung adenocarcinomas from Chinese male and female never-smokers.中国男性和女性非吸烟肺腺癌患者中表皮生长因子受体（EGFR）激酶结构域突变率相当。
Acta Pharmacol Sin. 2010 May;31(5):647-8. doi: 10.1038/aps.2010.43. Epub 2010 Apr 26.
6
Concurrent inhibition of NF-kappaB, cyclooxygenase-2, and epidermal growth factor receptor leads to greater anti-tumor activity in pancreatic cancer.同时抑制 NF-κB、环氧化酶-2 和表皮生长因子受体可增强胰腺癌的抗肿瘤活性。
J Cell Biochem. 2010 May;110(1):171-81. doi: 10.1002/jcb.22523.
7
Lung cancer cell lines: Useless artifacts or invaluable tools for medical science?肺癌细胞系：医学科学中无用的人工制品还是宝贵的工具？
Lung Cancer. 2010 Jun;68(3):309-18. doi: 10.1016/j.lungcan.2009.12.005. Epub 2010 Jan 15.
8
Cancer statistics, 2009.2009年癌症统计数据。
CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49. doi: 10.3322/caac.20006. Epub 2009 May 27.
9
KRAS mutations in non-small cell lung cancer.非小细胞肺癌中的KRAS突变
Proc Am Thorac Soc. 2009 Apr 15;6(2):201-5. doi: 10.1513/pats.200809-107LC.
10
Ethnic differences in drug metabolism and toxicity from chemotherapy.化疗药物代谢及毒性方面的种族差异。
Expert Opin Drug Metab Toxicol. 2009 Mar;5(3):243-57. doi: 10.1517/17425250902800153.
Zotero 插件