Lal R B, Kumaraswami V, Steel C, Nutman T B
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Am J Trop Med Hyg. 1990 Jan;42(1):56-64. doi: 10.4269/ajtmh.1990.42.56.
The immunosuppressive effect of Brugia malayi antigen (BmA) on phytohemagglutinin (PHA) driven T cell proliferation was evaluated in patients with filariasis (n = 14) and compared to control individuals (n = 12). When peripheral blood lymphocytes were co-cultured with BmA and PHA, BmA markedly suppressed the T cell proliferative response to PHA in both filarial patients and control individuals in a dose-dependent manner. The suppression resulted neither from any direct toxicity of BmA nor from nonspecific absorption of the PHA mitogenic activity by BmA. The major suppressive component appears to be phosphocholine (PC), an immunodominant molecule present in abundance on filarial parasites and on circulating filarial antigen. Both purified PC as well as PC-containing antigens affinity purified from BmA were capable of suppressing the proliferative responses of co-cultured autologous lymphocytes to PHA. The suppressive activity was not abolished by mitomycin-C treatment and was greater in patients with filariasis than in normal controls, suggesting that levels of PC-containing antigens determines the magnitude of the suppressive effect of PC-antigen. Further, as induction of the suppressive activity was completely abrogated when antigen pre-treated cells were T cell-depleted, the suppressive effect appears to be mediated primarily by T cells.
在丝虫病患者（n = 14）中评估了马来布鲁线虫抗原（BmA）对植物血凝素（PHA）驱动的T细胞增殖的免疫抑制作用，并与对照个体（n = 12）进行比较。当外周血淋巴细胞与BmA和PHA共同培养时，BmA以剂量依赖性方式显著抑制丝虫病患者和对照个体中T细胞对PHA的增殖反应。这种抑制既不是由BmA的任何直接毒性引起的，也不是由BmA对PHA促有丝分裂活性的非特异性吸收引起的。主要的抑制成分似乎是磷酸胆碱（PC），它是一种在丝虫寄生虫和循环丝虫抗原上大量存在的免疫显性分子。从BmA中亲和纯化的纯化PC以及含PC的抗原都能够抑制共培养的自体淋巴细胞对PHA的增殖反应。丝裂霉素-C处理不会消除这种抑制活性，并且在丝虫病患者中比正常对照中更强，这表明含PC抗原的水平决定了PC抗原的抑制作用的大小。此外，当抗原预处理的细胞去除T细胞时，抑制活性的诱导完全被消除，因此抑制作用似乎主要由T细胞介导。
