Miller J C, Wang Y, Filer D
Millhauser Laboratories of the Department of Psychiatry, New York University School of Medicine, New York 10016.
Biochem Biophys Res Commun. 1990 Jan 15;166(1):109-12. doi: 10.1016/0006-291x(90)91917-h.
A rat brain cDNA library constructed in lambda ZAP II was screened with three oligonucleotide probes based on the reported coding region of the D2 receptor gene, RGB-2. A complete cDNA clone, D2(8)-1, showing positive signals with the three probes was subsequently identified by restriction analysis and dideoxy sequence analysis to be a variant of the RGB-2 gene. Comparison of the two genes revealed almost complete homology except that D2(8)-1 contains an 87 bp insert within the protein coding region and 265 additional nucleotides 5' upstream from the 5' end reported for RGB-2. It is suggested that at least two mRNA species encoding for D2 receptors exist in rat brain, possibly resulting from alternative splicing of RNA.
用基于已报道的D2受体基因RGB - 2编码区的三种寡核苷酸探针筛选构建于λZAP II载体中的大鼠脑cDNA文库。随后通过限制性分析和双脱氧序列分析鉴定出一个完整的cDNA克隆D2(8)-1，它与这三种探针均呈阳性信号，是RGB - 2基因的一个变体。两个基因的比较显示几乎完全同源，只是D2(8)-1在蛋白质编码区内含有一个87 bp的插入片段，并且在RGB - 2报道的5'端上游5'还有265个额外的核苷酸。提示大鼠脑中至少存在两种编码D2受体的mRNA，可能是RNA可变剪接的结果。
