King's College London Dental Institute, Microbiology, Guy's Campus, London, SE1 9RT, UK.
Arch Microbiol. 2011 Jun;193(6):407-17. doi: 10.1007/s00203-011-0689-6. Epub 2011 Mar 5.
We show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. Numerous strains of Actinobacteria (Brevibacterium, Gordonia, Leifsonia, Microbacterium, Micrococcus, Rhodococcus) and Proteobacteria (Achromobacter, Klebsiella, Methylobacterium, Pseudomonas, Ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. These strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydrogenases), and the assimilation of one-carbon compounds by the serine pathway (hydroxypyruvate reductase). Methylotrophic growth of the strains was enhanced by the addition of glass beads to cultures, suggesting that they may naturally occur in biofilms in the mouth. This is the first report of Gordonia, Leifsonia, and Rhodococcus being present in the mouth and of the unequivocal demonstration for the first time of the methylotrophic potential of strains of Gordonia, Leifsonia, and Microbacterium.
我们表明，具有甲基营养潜能的细菌在人类口腔微生物群中无处不在。从放线菌（Brevibacterium、Gordonia、Leifsonia、Microbacterium、Micrococcus、Rhodococcus）和变形菌（Achromobacter、Klebsiella、Methylobacterium、Pseudomonas、Ralstonia）中分离出了许多菌株，并对其中的十一个属中的每一个属的一个菌株进行了详细研究。这些菌株表达了与甲基营养代谢（甲醇、甲胺和甲酸盐脱氢酶）以及丝氨酸途径（羟丙酮酸还原酶）中一碳化合物同化相关的酶。在培养物中添加玻璃珠可增强菌株的甲基营养生长，表明它们可能自然存在于口腔生物膜中。这是首次报道 Gordonia、Leifsonia 和 Rhodococcus 存在于口腔中，也是首次明确证明 Gordonia、Leifsonia 和 Microbacterium 菌株具有甲基营养潜能。
