抗肌醇聚糖抗体可选择性地阻断完整的BC3H1细胞中胰岛素的某些作用。
Anti-inositolglycan antibodies selectively block some of the actions of insulin in intact BC3H1 cells.
作者信息
Romero G, Gámez G, Huang L C, Lilley K, Luttrell L
机构信息
Department of Pharmacology, University of Virginia, Charlottesville 22908.
出版信息
Proc Natl Acad Sci U S A. 1990 Feb;87(4):1476-80. doi: 10.1073/pnas.87.4.1476.
Abstract
We have studied the mechanism of generation of insulin mediators by using specific antibodies raised against the oligosaccharide anchor of membrane proteins. These antibodies (i) block the in vitro effects of purified insulin mediators and (ii) block the insulin-induced stimulation of pyruvate dehydrogenase in intact BC3H1 myocytes but not insulin-stimulated glucose uptake, generation of diacylglycerol, or generation of insulin mediators. When added to intact cells in the presence of insulin, these antibodies induce the accumulation of insulin mediator activity in the extracellular medium. We therefore conclude that these anti-inositolglycan antibodies block some of the effects of insulin by inhibiting the uptake of specific insulin mediators generated outside the cell.
摘要
我们利用针对膜蛋白寡糖锚制备的特异性抗体，研究了胰岛素介质的产生机制。这些抗体：（i）阻断纯化胰岛素介质的体外效应；（ii）阻断胰岛素诱导的完整BC3H1肌细胞中丙酮酸脱氢酶的刺激作用，但不阻断胰岛素刺激的葡萄糖摄取、二酰基甘油的生成或胰岛素介质的生成。当在胰岛素存在的情况下添加到完整细胞中时，这些抗体诱导细胞外介质中胰岛素介质活性的积累。因此，我们得出结论，这些抗肌醇聚糖抗体通过抑制细胞外产生的特定胰岛素介质的摄取，阻断了胰岛素的某些效应。
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