GlaxoSmithKline Consumer Healthcare, Weybridge, UK.
Int Dent J. 2013 Dec;63 Suppl 2(Suppl 2):39-47. doi: 10.1111/idj.12072.
To compare the effect of 40 seconds versus 2 minutes brushing on saliva and dental biofilm fluid fluoride in children ages 4-5 years over 1 hour.
This was a single-blind, cross-over, randomised, two-period clinical study in healthy children. Three days before the start of each treatment subjects received a thorough brushing and then refrained from all oral hygiene procedures. At treatment visits, after collecting baseline biofilm and saliva samples, staff brushed the occlusal surfaces of the subject's posterior teeth with a pea-sized amount (0.25 g) of NaF/silica toothpaste for the randomised time. Samples were taken at 5 minutes, 15 minutes, 30 minutes and 60 minutes after brushing and analysed for fluoride using a microanalytical methodology. There was a minimum 4-day washout period between treatments.
Log changes from baseline biofilm fluid and saliva fluoride were statistically significant (P < 0.05) for both brushing times at all post-brushing time-points [except 60 minutes saliva where P = 0.06 (t-test)]. Statistically significantly greater ln-AUC (area under the curve) was found for biofilm fluid and salivary fluoride after brushing for 2 minutes compared with brushing for 40 seconds over the 1-hour test period. There was a statistically significantly higher concentration of fluoride in the log change from baseline saliva levels after 5, 15, 30 and 60 minutes for the 2-minute brushing time compared with 40 seconds brushing time. There was no statistically significant difference in concentration of log change from baseline fluoride levels in biofilm fluid at each individual time-point (5, 15, 30 and 60 minutes) for the 2-minute brushing time compared with the 40-second brushing time, but significant differences were observed for 15, 30 and 60 minutes in favour of 2-minute brushing time when log biofilm fluid value was analysed.
The findings provide further evidence for the benefits of increased duration of brushing with respect to fluoride delivery.
比较 40 秒与 2 分钟刷牙对 4-5 岁儿童唾液和牙菌斑氟化物的影响，持续 1 小时。
这是一项单盲、交叉、随机、两周期的临床研究，纳入健康儿童。在每次治疗前 3 天，受试者接受彻底刷牙，然后避免所有口腔卫生措施。在治疗就诊时，在收集基线牙菌斑和唾液样本后，工作人员用豌豆大小（0.25 克）的 NaF/二氧化硅牙膏为随机时间刷牙，刷磨牙的咬合面。在刷牙后 5 分钟、15 分钟、30 分钟和 60 分钟时采集样本，并使用微量分析方法检测氟化物。两种处理之间至少有 4 天的洗脱期。
两种刷牙时间在所有刷牙后时间点（60 分钟唾液除外，P=0.06（t 检验））的牙菌斑氟化物和唾液氟化物的基线 log 变化均具有统计学意义（P<0.05）。在 1 小时测试期间，与 40 秒刷牙相比，2 分钟刷牙的牙菌斑氟化物和唾液氟化物的 ln-AUC（曲线下面积）明显更大。与 40 秒刷牙相比，2 分钟刷牙后，5、15、30 和 60 分钟的唾液基线 log 变化的氟浓度明显更高。在每个单独的时间点（5、15、30 和 60 分钟），2 分钟刷牙的牙菌斑氟化物基线 log 变化的氟浓度与 40 秒刷牙无统计学差异，但当分析 log 牙菌斑氟化物值时，15、30 和 60 分钟时，2 分钟刷牙时间明显有利于 2 分钟刷牙。
研究结果进一步证明了刷牙时间延长对氟化物输送的益处。
