Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, Xi'an 710032, People's Republic of China.
J Pharm Biomed Anal. 2011 Dec 5;56(4):820-4. doi: 10.1016/j.jpba.2011.07.016. Epub 2011 Jul 23.
A simple, accurate and reliable method for the simultaneous separation and determination of 9 active components (danshensu, protocatechuic acid, protocatechuic aldehyde, caffeic acid, rosmarinic acid, salvianolic acid B, paeonol, paeoniflorin and gallic acid) in traditional Chinese medicinal preparation ShuangDan (SD) oral liquid was developed using reverse phase high-performance liquid chromatography (RP-HPLC) coupled with photodiode array (PDA) detection. The chromatographic separation was performed on a SinoChrom ODS-BP C(18) column with gradient elution using methanol (A) and 3% glacial acetic acid aqueous solution (B) at a flow rate of 1.0mLmin(-1), and with a PDA detection. Good linear behaviors over the investigated concentration ranges were observed with the values of r(2) higher than 0.9992 for all the analytes. The recoveries and relative standard deviation (RSD), measured at three concentration levels, varied from 98.21% to 101.82% and 0.07% to 1.37%, respectively. The proposed method enables the simultaneous identification and determination of 9 active components in a single run for the quality control of ShuangDan oral liquid.
建立了一种同时分离和测定复方丹参口服液中 9 种有效成分（丹参素、原儿茶酸、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸 B、丹皮酚、芍药苷和没食子酸）的反相高效液相色谱法（RP-HPLC）-光电二极管阵列（PDA）检测法。采用 SinoChrom ODS-BP C（18）柱，以甲醇（A）和 3%冰醋酸水溶液（B）为流动相，梯度洗脱，流速为 1.0mL/min，在 PDA 检测下进行色谱分离。所有分析物在考察的浓度范围内均表现出良好的线性行为，相关系数（r（2））均高于 0.9992。在三个浓度水平下，回收率和相对标准偏差（RSD）的变化范围分别为 98.21%至 101.82%和 0.07%至 1.37%。该方法可在单次运行中同时鉴定和测定复方丹参口服液中的 9 种有效成分，可用于其质量控制。
