Center for Environmental Research and Technology and Chemical and Environmental Engineering Department, University of California, 1084 Columbia Ave., Riverside, CA 92507, USA.
Bioresour Technol. 2011 May;102(10):6295-8. doi: 10.1016/j.biortech.2011.02.016. Epub 2011 Mar 3.
Bovine serum albumin (BSA) was applied as a model non-catalytic protein to enzymatic hydrolysis of Avicel and dilute acid pretreated corn stover at different reaction conditions to improve the understanding of its ability to enhance cellulose hydrolysis. Addition of BSA improved the 72 h hydrolysis yields in shake flasks by up to 26% for both substrates by reducing de-activation of the exoglucanases and by facilitating reductions in particle size and crystallinity during a magnetically stirred pre-incubation step. The enzyme stabilizing effect of BSA addition was most striking for batch hydrolysis in a stirred tank reactor, with glucose yields increasing by 76% after 72 h for Avicel and by 40% after 145 h for corn stover. Application of BSA to continuous hydrolysis for a mean residence time of 24h gave 33% and 40% higher glucose yields for corn stover and Avicel compared to the controls.
牛血清白蛋白（BSA）被用作一种模型非催化蛋白，用于在不同反应条件下对纤维素酶水解 Avicel 和稀酸预处理的玉米秸秆进行酶解，以加深对其增强纤维素水解能力的理解。添加 BSA 可通过减少外切葡聚糖酶的失活并在磁力搅拌预孵育步骤中促进粒径和结晶度的降低，使摇瓶中两种底物的 72 小时水解产率提高高达 26%。在搅拌罐反应器中分批水解时，BSA 添加的酶稳定作用最为显著，Avicel 的葡萄糖产率在 72 小时后增加了 76%，玉米秸秆的葡萄糖产率在 145 小时后增加了 40%。对于平均停留时间为 24 小时的连续水解，与对照相比，BSA 的应用使玉米秸秆和 Avicel 的葡萄糖产率分别提高了 33%和 40%。
