Unitat de Biocatàlisi Aplicada associada al IQAC (CSIC-UAB), Escola d'Enginyeria, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Bioresour Technol. 2011 May;102(10):5602-8. doi: 10.1016/j.biortech.2011.02.029. Epub 2011 Mar 4.
For safe biosolid-land-applying, sludge should be contaminant-free. However, it may contain important amounts of micropollutants, not removed in the wastewater-treatment-processes. An alternative treatment with the fungus Trametes versicolor was applied in sterile solid-phase systems consisting of sludge and a lignocellulosic substrate. Fungal colonization and activity were demonstrated during the process, according to monitoring of ergosterol, laccase activity and the naproxen-degradation test (ND24). Fourteen out of 43 analyzed pharmaceuticals were found in the raw sludge. After treatment, phenazone, bezafibrate, fenofibrate, cimetidine, clarithromycin, sulfamethazine and atenolol were completely removed, while removals between 42% and 80% were obtained for the remaining pharmaceuticals. Toxicological analyses (Daphnia magna, Vibrio fischeri and seed germination) showed an important reduction in sludge toxicity after treatment. Results suggest that a solid-phase treatment with T. versicolor may reduce the ecotoxicological impact of micropollutants present in sewage sludge. This is the first report of a fungal-approach for elimination of emerging pollutants from biosolids.
为了安全地将生物固体施用于土地，污泥应不含污染物。然而，它可能含有大量未在废水处理过程中去除的微量污染物。一种替代处理方法是使用木腐真菌彩绒革盖菌在由污泥和木质纤维素基质组成的无菌固相系统中进行。根据麦角固醇、漆酶活性和萘普生降解试验（ND24）的监测，证明了真菌的定殖和活性。在原始污泥中发现了 43 种分析药物中的 14 种。处理后，苯并恶唑、苯扎贝特、非诺贝特、西咪替丁、克拉霉素、磺胺甲恶唑和阿替洛尔被完全去除，而其余药物的去除率在 42%至 80%之间。毒理学分析（大型溞、发光菌和种子发芽）表明，处理后污泥的毒性显著降低。结果表明，彩绒革盖菌的固相处理可能会降低污水污泥中存在的环境污染物的生态毒性影响。这是首例报道真菌方法从生物固体中消除新兴污染物的研究。
