Department of Histology, University of Granada, Granada, Spain.
J Histochem Cytochem. 2011 Mar;59(3):270-7. doi: 10.1369/0022155410398001. Epub 2011 Jan 12.
For the histopathologic diagnosis of melanocytic lesions, it could be necessary to identify the melanin pigment because its visualization is unspecific with hematoxylin-eosin (HE). The Fontana-Masson (FM) technique is used in histopathology in this type of lesion, which allows the identification of the pigment, but it loses all the morphologic parameters. The authors describe a modification to the FM method, for the evaluation of the morphology, the argentaffin reaction of the melanin, and collagens fibers of the extracellular matrix simultaneously, for which they have developed the Fontana-Masson picrosirius (FMPS) method. Biopsies of different melanocytic lesions were used for the performance of the HE, FM, and FMPS methods. The pixel intensity of the reaction for melanin, collagen, and epithelium was determined with ImageJ software. The FMPS method allows the evaluation of morphological characteristics, identifying the melanin pigment and collagen fibers with high intensity simultaneously. This method does not differ significantly from FM in the identification of melanin, maintaining its sensitivity and specificity. In addition, it does not differ in the demonstration of the morphology with HE. However, FMPS is significantly superior in the identification of collagen fibers. The FMPS method combines morphological and histochemical parameters that could be useful in the study of pigmented lesions of melanocytic origin.
对于黑素细胞病变的组织病理学诊断，可能需要识别黑色素，因为苏木精-伊红（HE）对其可视化不具有特异性。Fontana-Masson（FM）技术在组织病理学中用于这种类型的病变，可以识别色素，但会失去所有形态学参数。作者描述了一种 FM 方法的改进，用于同时评估形态、黑色素的嗜银反应和细胞外基质的胶原纤维，为此他们开发了 Fontana-Masson 皮尔斯（FMPS）方法。使用不同的黑素细胞病变活检来进行 HE、FM 和 FMPS 方法的检测。使用 ImageJ 软件确定反应的黑色素、胶原和上皮细胞的像素强度。FMPS 方法可以评估形态特征，同时识别高强度的黑色素和胶原纤维。该方法在黑色素的识别方面与 FM 无显著差异，保持其敏感性和特异性。此外，在 HE 显示形态方面也没有差异。然而，在识别胶原纤维方面，FMPS 具有显著优势。FMPS 方法结合了形态学和组织化学参数，这在研究黑素细胞来源的色素性病变时可能很有用。
