Warkel R L, Luna L G, Helwig E B
Am J Clin Pathol. 1980 Jun;73(6):812-5. doi: 10.1093/ajcp/73.6.812.
The Warthin-Starry argyrophilic silver stain peformed at pH 3.2 is a highly effective method for the demonstration of melanin in tissue sections. Eighty-five melanocytic tumors were studied using the Warthin-Starry and the Fontana-Masson procedures. The Warthin-Starry technic stained a greater number of cells in 68% of the tumors, with no loss of cellular detail. Twenty-three per cent of the tumors that appeared amelanotic on hematoxylin-and-eosin-stained sections were positive for melanin with the Warthin-Starry stain, but negative with the Fontana-Masson technic. The Warthin-Starry stain is more sensitive and specific for melanin than is the Fontana-Masson procedure.
在pH 3.2条件下进行的Warthin-Starry嗜银银染色是在组织切片中显示黑色素的一种高效方法。使用Warthin-Starry和Fontana-Masson方法对85个黑素细胞瘤进行了研究。Warthin-Starry技术在68%的肿瘤中染色的细胞数量更多，且细胞细节未丢失。在苏木精-伊红染色切片上表现为无黑色素的肿瘤中，23%的肿瘤用Warthin-Starry染色显示黑色素呈阳性，但用Fontana-Masson技术染色呈阴性。Warthin-Starry染色对黑色素的敏感性和特异性高于Fontana-Masson方法。
