Pisetsky D S, Jelinek D F, McAnally L M, Reich C F, Lipsky P E
Medical Service, Durham Veterans Administration Hospital, North Carolina 27705.
J Clin Invest. 1990 Mar;85(3):899-903. doi: 10.1172/JCI114517.
To investigate the repertoire of autoantibodies in humans, anti-DNA and rheumatoid factor (RF) production in vitro was assessed in cultures of adult peripheral blood B cells and neonatal umbilical venous blood B cells. B cells were stimulated under various culture conditions, using an immobilized monoclonal anti-CD3 antibody and adult T cells or Staphylococcus aureus (SA) in the presence or absence of adult T cells or factors derived from mitogen-stimulated adult T cells as polyclonal B cell activators. Total IgM, as well as IgM anti-DNA and RF, were assessed by ELISA. Total IgM production was induced from adult and neonatal B cells with SA plus T cell factors, as well as anti-CD3-stimulated T cells. RF was induced from adult and cord blood B cells by either mode of stimulation, whereas significant anti-DNA production was observed only when B cells were stimulated with anti-CD3-activated T cells. These results confirm the presence of B cell precursors for autoantibodies in the preimmune as well as normal adult repertoire, and indicate that the production of anti-DNA and RF appears to be regulated independently.
为研究人类自身抗体库，在成人外周血B细胞和新生儿脐静脉血B细胞培养物中评估了体外抗DNA和类风湿因子（RF）的产生。使用固定化单克隆抗CD3抗体和成人T细胞或金黄色葡萄球菌（SA），在有或无成人T细胞或来自丝裂原刺激的成人T细胞的因子作为多克隆B细胞激活剂的情况下，在各种培养条件下刺激B细胞。通过酶联免疫吸附测定（ELISA）评估总IgM以及IgM抗DNA和RF。SA加T细胞因子以及抗CD3刺激的T细胞可诱导成人和新生儿B细胞产生总IgM。两种刺激方式均可诱导成人和脐血B细胞产生RF，而仅当B细胞用抗CD3激活的T细胞刺激时才观察到显著的抗DNA产生。这些结果证实了在免疫前以及正常成人抗体库中存在自身抗体的B细胞前体，并表明抗DNA和RF的产生似乎是独立调节的。
